Team:UNIPV-Pavia/Methods Materials/LB

From 2009.igem.org

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'''Yeast extract '''<hr color='white' width='70%'>
'''Yeast extract '''<hr color='white' width='70%'>
'''ddH2O'''<hr color='white' width='70%'>
'''ddH2O'''<hr color='white' width='70%'>
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'''Agar'''
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'''Agar''' </font>
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Revision as of 13:19, 30 June 2009

EthanolPVanimation.gif



Protocols



LB medium

(Estimated time: 10 min + 4 hours of autoclavation and cooling)

  • For a final volume of 1 l, put:
    • 10 g NaCl
    • 10 g BactoTryptone
    • 5 g Yeast extract
    • 15 g Agar (only for LB plates)
  • into 1 l of ddH2O.
  • Autoclave the solution.
  • Let it cool to ~40-45°C
  • Add the proper antibiotic if needed.
  • ONLY FOR PLATES:
    • Pour an homogenous layer of agar LB into Petri plates under the hood.
    • Let agar LB polymerase.
  • Cover and store at +4°C.
  • Always check for contaminations before using!










Clean bottle or flask
NaCl
BactoTryptone
Yeast extract
ddH2O

Agar





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