Team:UNIPV-Pavia/Methods Materials/Measurement

From 2009.igem.org

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     <font face="Pristina" size="50" ><b>Measurements</b></font>
     <font face="Pristina" size="50" ><b>Measurements</b></font>
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A preliminar study was done on the instrument TECAN Infinite F200, a multifunctional microplate filter-based reader described in [https://2009.igem.org/Team:UNIPV-Pavia/Methods_Materials/TECAN#TECAN_Infinite_F200 Instruments], to understand the behaviour of the measurements of absorbance and fluorescence intensity in different situations.
A preliminar study was done on the instrument TECAN Infinite F200, a multifunctional microplate filter-based reader described in [https://2009.igem.org/Team:UNIPV-Pavia/Methods_Materials/TECAN#TECAN_Infinite_F200 Instruments], to understand the behaviour of the measurements of absorbance and fluorescence intensity in different situations.
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The results of the experiments, grouped by argoments, are described here, if you need more informations about a single experiment, you can find the detailed protocol at [http://aimed11.unipv.it/iGEM2009 Download Protocols].
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The results of this experimental setup, grouped by arguments, are described here. If you need more information about a single experiment, you can find the detailed protocol at <html><a href="http://aimed11.unipv.it/iGEM2009/TestTECANInfiniteF200/" target="_blank">Download Protocols</a></html>.
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*[[Team:UNIPV-Pavia/Methods_Materials/Absorbance#Absorbance and volume|Absorbance and volume]]
*[[Team:UNIPV-Pavia/Methods_Materials/Absorbance#Absorbance and volume|Absorbance and volume]]
*[[Team:UNIPV-Pavia/Methods_Materials/Absorbance#Absorbance and dilutions in liquid growth medium|Absorbance and dilutions in liquid growth medium]]
*[[Team:UNIPV-Pavia/Methods_Materials/Absorbance#Absorbance and dilutions in liquid growth medium|Absorbance and dilutions in liquid growth medium]]
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*[[Team:UNIPV-Pavia/Methods_Materials/Absorbance#Different protocols for different growth curves|Different protocols for different growth curves]]
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*[[Team:UNIPV-Pavia/Methods_Materials/Absorbance#Growth ambients in comparison|Growth ambients in comparison]]
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'''FLUORESCENCE''':
'''FLUORESCENCE''':
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• In long experiments do not use the well on the frame, but fill them with water or growth medium to maintain the temperature homogenous in the plate;<br><br>
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• In long experiments do not use the wells on the frame, but fill them with water or growth medium to maintain the temperature homogenous in the plate;<br><br>
   
   
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• in long experiments cover the plate with the lid to limit the evaporation;<br><br>
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• in long experiments cover the plate with the lid to limit the evaporation phenomenon;<br><br>
• use the O.D. measurements to quantify bacterial growth, regardless of the volume in the well;<br><br>
• use the O.D. measurements to quantify bacterial growth, regardless of the volume in the well;<br><br>
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• do not dispense water if you perform fluorescence measurements;<br><br>
• do not dispense water if you perform fluorescence measurements;<br><br>
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• work with volumes not larger than 200 μl and prefer the M9 medium, if you perform fluorescence measurements;<br><br>
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• work with volumes not larger than 200 μl and prefer the M9 medium if you perform fluorescence measurements;<br><br>
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• in long experiments, if you perform fluorescence measurements on fluorescent coltures, always subtract the blank value, to correct the descending evolution of the fluorescence during the incubation.
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• in long experiments, if you perform fluorescence measurements on fluorescent cultures, always subtract the blank value individually on every sampling to correct the descending evolution of the fluorescence during the incubation.
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Latest revision as of 16:06, 19 October 2009

EthanolPVanimation.gif



Measurements


A preliminar study was done on the instrument TECAN Infinite F200, a multifunctional microplate filter-based reader described in Instruments, to understand the behaviour of the measurements of absorbance and fluorescence intensity in different situations.

The results of this experimental setup, grouped by arguments, are described here. If you need more information about a single experiment, you can find the detailed protocol at Download Protocols.


Studies:

ABSORBANCE:


FLUORESCENCE:

EVAPORATION:


CONCLUSIONS: EXPERIMENTAL SET UP AND MEASUREMENT METHODS

• In long experiments do not use the wells on the frame, but fill them with water or growth medium to maintain the temperature homogenous in the plate;

• in long experiments cover the plate with the lid to limit the evaporation phenomenon;

• use the O.D. measurements to quantify bacterial growth, regardless of the volume in the well;

• do not dispense water if you perform fluorescence measurements;

• work with volumes not larger than 200 μl and prefer the M9 medium if you perform fluorescence measurements;

• in long experiments, if you perform fluorescence measurements on fluorescent cultures, always subtract the blank value individually on every sampling to correct the descending evolution of the fluorescence during the incubation.

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