Team:UNIPV-Pavia/Methods Materials/Measurement

From 2009.igem.org

(Difference between revisions)
Line 67: Line 67:
<tr bgcolor="royalblue">
<tr bgcolor="royalblue">
<td><font color="white">
<td><font color="white">
-
• In long experiments do not use the well on the frame, but fill them with water or growing medium to mantain the temperature homogenous in the plate;
+
• In long experiments do not use the well on the frame, but fill them with water or growing medium to maintain the temperature homogenous in the plate;<br><br>
   
   
•   
•   

Revision as of 21:53, 12 October 2009

EthanolPVanimation.gif



Measurements


A preliminar study was done on the instrument TECAN Infinite F200, a multifunctional microplate filter-based reader described in Instruments, to understand the behaviour of the measurements of absorbance and fluorescence intensity in different situations.

The results of the experiments, grouped by argoments, are described here, if you need more informations about a single experiment, you can find the detailed protocol at Download Protocols.


Studies:

ABSORBANCE:

FLUORESCENCE:

EVAPORATION:


CONCLUSIONS: THE BEST EXPERIMENTAL SET UP
• In long experiments do not use the well on the frame, but fill them with water or growing medium to maintain the temperature homogenous in the plate;