Team:UNIPV-Pavia/Methods Materials/PlasmidExtraction

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Latest revision as of 10:28, 21 October 2009

Protocols

Plasmid extraction

(Estimated time: 1 hour)

Follow QIAGEN standard protocol: QIAGEN QIAprep Spin Miniprep protocol

INGREDIENTS:

5 ml or 9 ml (for higher yields) overnight mini-culture

QIAGEN QIAprep Spin Miniprep Kit

@@ Line 1: / Line 1: @@
-<h1>Plasmid extraction</h1>
+<html>
-''(Estimated time: 3 hours if you use QIAGEN Plasmid Mini Kit; 1 hour if you use QIAGEN QIAprep Spin Miniprep Kit)''
+<table width="100%">
+  <tr>
+	<td align="center">
+	<img width="100%" height="180px" src="https://static.igem.org/mediawiki/2009/d/de/UNIPV_title_M%26M.jpg" ></img>
+	</td>
+  </tr>
+</table>
+</html>
+<table width="100%">
+  <tr>
+    <td valign="top" width= "23%" rowspan="2">
+{{UNIPV-Pavia/Menu}}
+    </td>
+<html>
+    <td width="50%"></td>
+    <td align="center"><br><bR>
+     <font face="Pristina" size="50" ><b>Protocols</b></font>
+    </td>
+    <td width="50px"></td>
+</html>
+  </tr>
+<tr>
+  <td> {{UNIPV-Pavia/Protocols}}</td>
+</tr>
+</table>
+=='''Plasmid extraction'''==
+''(Estimated time: 1 hour)''
 <br>
 <br>
-'''Materials needed:'''
+<table width='80%'><tr><td>
-*'''5 ml or 9 ml (for higher yields) overnight mini-culture
+*Follow QIAGEN standard protocol: [http://www.biochem.arizona.edu/AZ-START/graphics/QIAprep_Miniprep_Handbook.pdf QIAGEN QIAprep Spin Miniprep protocol]
-*'''QIAGEN QIAprep Spin Miniprep Kit'''
+<br><br><br><br><br><br><br><br>
-<br>
+<br></td><td align='center' valign='top'  width='300px'>
-*Follow QIAGEN standard protocol: [http://www1.qiagen.com/literature/render.aspx?id=370&tp=4 QIAGEN QIAprep Spin Miniprep protocol]
-<br>
+<table border='1'>
+<tr bgcolor='yellow'><td>INGREDIENTS:
+</td></tr>
+<tr><td>
+<font class='label'>
+'''5 ml or 9 ml (for higher yields) overnight mini-culture <hr color='white' width='70%'>
+'''QIAGEN QIAprep Spin Miniprep Kit'''</font>
+</td></tr></table><br><br><br><br>