Team:UNIPV-Pavia/Methods Materials/Resuspension

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(→DNA resuspension from iGEM 2009 plates)
 
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     <font face="Pristina" size="50" ><b>Protocols</b></font>
     <font face="Pristina" size="50" ><b>Protocols</b></font>
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== '''LB medium''' ==
 
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''(Estimated time: 10 min + 4 hours of autoclavation and cooling)''
 
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<br>
 
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<br>
 
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'''Materials needed:'''
 
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*'''NaCl'''
 
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*'''BactoTryptone'''
 
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*'''Yeast extract '''
 
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*'''ddH2O'''
 
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*'''Agar'''
 
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*'''Clean bottle or E-flask'''
 
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<br>
 
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*For a final volume of 1 l, put:
 
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**10 g NaCl
 
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**10 g BactoTryptone
 
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**5 g Yeast extract
 
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**15 g Agar (only for LB plates)
 
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*into 1 l of ddH2O.
 
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*Autoclave the solution.
 
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*Let it cool to ~40-45°C
 
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*Add the proper antibiotic if needed.
 
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*ONLY FOR PLATES:
 
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**Pour an homogenous layer of agar LB into Petri plates under the hood.
 
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**Let agar LB polymerase.
 
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*Cover and store at +4°C.
 
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*Always check for contaminations before using!
 
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== '''DNA resuspension from iGEM 2009 plates''' ==  
== '''DNA resuspension from iGEM 2009 plates''' ==  
''(Estimated time: 30 min)''
''(Estimated time: 30 min)''
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<br>
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'''Materials needed:'''
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*'''iGEM 2009 plates'''
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*'''PC with internet connection :-)'''
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*'''ddH20'''
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*'''0.5 ml sterile Eppendorf tubes'''
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<br>
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*Find the correct position of the desired DNA from the iGEM 2009 plates
*Find the correct position of the desired DNA from the iGEM 2009 plates
*Resuspend with ddH20 and transfer in an Eppendorf tubes
*Resuspend with ddH20 and transfer in an Eppendorf tubes
*Store at -20°C
*Store at -20°C
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<tr bgcolor='yellow'><td>INGREDIENTS:
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'''iGEM 2009 plates'''<hr color='white' width='70%'>
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'''PC with internet connection :-)'''<hr color='white' width='70%'>
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'''ddH20'''<hr color='white' width='70%'>
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'''0.5 ml sterile Eppendorf tubes'''</font>
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Latest revision as of 10:26, 21 October 2009

EthanolPVanimation.gif



Protocols


DNA resuspension from iGEM 2009 plates

(Estimated time: 30 min)

  • Find the correct position of the desired DNA from the iGEM 2009 plates
  • Resuspend with ddH20 and transfer in an Eppendorf tubes
  • Store at -20°C














INGREDIENTS:

iGEM 2009 plates
PC with internet connection :-)
ddH20

0.5 ml sterile Eppendorf tubes





Retrieved from "http://2009.igem.org/Team:UNIPV-Pavia/Methods_Materials/Resuspension"