Team:UNIPV-Pavia/Methods Materials/Resuspension

From 2009.igem.org

(Difference between revisions)
(DNA resuspension from iGEM 2009 plates)
 
(11 intermediate revisions not shown)
Line 1: Line 1:
-
{{UNIPV-Pavia/StyleCss}}
 
<html>
<html>
-
<head>
+
 
-
<link rel="stylesheet" type="text/css" href="http://bioinfo.unipv.it/igem/templates/SpryAssets/SpryMenuBar.js" title="Stile"/>
+
-
</head>
+
-
<body>
+
<table width="100%">
<table width="100%">
   <tr>
   <tr>
<td align="center">
<td align="center">
-
<img width="100%" height="180px" src="https://static.igem.org/mediawiki/2009/c/c2/UNIPV_title_MandM.jpg" ></img>
+
<img width="100%" height="180px" src="https://static.igem.org/mediawiki/2009/d/de/UNIPV_title_M%26M.jpg" ></img>
</td>
</td>
   </tr>
   </tr>
</table>
</table>
-
</body>
+
 
</html>
</html>
Line 25: Line 21:
     <font face="Pristina" size="50" ><b>Protocols</b></font>
     <font face="Pristina" size="50" ><b>Protocols</b></font>
     </td>
     </td>
 +
    <td width="50px"></td>
</html>
</html>
   </tr>
   </tr>
Line 38: Line 35:
<br>
<br>
<br>
<br>
-
'''Materials needed:'''
+
<table width='80%'><tr><td>
-
*'''iGEM 2009 plates'''
+
-
*'''PC with internet connection :-)'''
+
-
*'''ddH20'''
+
-
*'''0.5 ml sterile Eppendorf tubes'''
+
-
<br>
+
*Find the correct position of the desired DNA from the iGEM 2009 plates
*Find the correct position of the desired DNA from the iGEM 2009 plates
*Resuspend with ddH20 and transfer in an Eppendorf tubes
*Resuspend with ddH20 and transfer in an Eppendorf tubes
*Store at -20°C
*Store at -20°C
 +
<br><br><br><br><br><br><br><br><br><br><br><br>
 +
<br></td><td align='center' valign='top'  width='300px'>
 +
 +
<table border='1'>
 +
<tr bgcolor='yellow'><td>INGREDIENTS:
 +
</td></tr>
 +
<tr><td>
 +
<font class='label'>
 +
'''iGEM 2009 plates'''<hr color='white' width='70%'>
 +
'''PC with internet connection :-)'''<hr color='white' width='70%'>
 +
'''ddH20'''<hr color='white' width='70%'>
 +
'''0.5 ml sterile Eppendorf tubes'''</font>
 +
</td></tr></table><br><br><br><br>
 +
</td></tr></table>

Latest revision as of 10:26, 21 October 2009

EthanolPVanimation.gif



Protocols


DNA resuspension from iGEM 2009 plates

(Estimated time: 30 min)

  • Find the correct position of the desired DNA from the iGEM 2009 plates
  • Resuspend with ddH20 and transfer in an Eppendorf tubes
  • Store at -20°C














INGREDIENTS:

iGEM 2009 plates
PC with internet connection :-)
ddH20

0.5 ml sterile Eppendorf tubes