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Team:UNIPV-Pavia/Methods Materials/Resuspension
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(New page: {{UNIPV-Pavia/Protocols}} <br> == '''DNA resuspension from iGEM 2009 plates''' == ''(Estimated time: 30 min)'' <br> <br> '''Materials needed:''' *'''iGEM 2009 plates''' *'''PC with intern...)
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(New page: {{UNIPV-Pavia/Protocols}} <br> == '''DNA resuspension from iGEM 2009 plates''' == ''(Estimated time: 30 min)'' <br> <br> '''Materials needed:''' *'''iGEM 2009 plates''' *'''PC with intern...)
Newer edit →
Revision as of 17:02, 22 June 2009
- LB medium
- DNA resuspension from iGEM 2009 plates
- Transformation
- X-Gal staining protocol for beta galactosidase (blue/white screening)
- Glycerol stocks
- Plasmid extraction
- BioBrick digestion with restriction enzymes
- Electrophoresis
- DNA gel extraction
- DNA precipitation with sodium acetate
- Ligation
- PCR
- Sensing ethanol concentration: our do-it-yourself kit
DNA resuspension from iGEM 2009 plates
(Estimated time: 30 min)
Materials needed:
- iGEM 2009 plates
- PC with internet connection :-)
- ddH20
- 0.5 ml sterile Eppendorf tubes
- Find the correct position of the desired DNA from the iGEM 2009 plates
- Resuspend with ddH20 and transfer in an Eppendorf tubes
- Store at -20°C
