Team:UNIPV-Pavia/Methods Materials/XGal

(Difference between revisions)

Latest revision as of 10:27, 21 October 2009

Protocols

(Estimated time: 20 min)

The principle is that X-Gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside) turns blue when reacts with beta-galactosidase.
Mix 20 ul X-Gal 20 mg/ml and 80 ul SOC and spread on LB+amp plates (X-Gal and DMF are toxic, use face-mask for your safety!!! X-Gal is light-sensitive, remember to keep it in the dark, when possible)
Incubate with 200 ul bacteria
Result: blue colonies express LacZ, while white colonies don't.

INGREDIENTS:

40 mg/ml X-gal in DMF

SOC medium

Pre-warmed LB agar plate

Face mask

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 *Result: blue colonies express LacZ, while white colonies don't.
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+<br></td><td align='center' valign='top'  width='300px'>
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-*'''40 mg/ml X-gal in DMF'''
+<font class='label'>
-*'''SOC medium'''
+'''40 mg/ml X-gal in DMF'''<hr color='white' width='70%'>
-*'''Pre-warmed LB agar plate'''
+'''SOC medium'''<hr color='white' width='70%'>
-*'''Face mask'''
+'''Pre-warmed LB agar plate'''<hr color='white' width='70%'>
-</td></tr></table></div><br><br><br><br>
+'''Face mask'''</font>
+</td></tr></table><br><br><br><br>
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