Team:UNIPV-Pavia/Protocols/Precipitation

From 2009.igem.org

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(→DNA precipitation with sodium acetate)
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<table class='in_centrifuge' border='1'>
<table class='in_centrifuge' border='1'>
<tr><td>
<tr><td>
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*'''Sodium acetate'''
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<font class='label'>
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*'''Absolute ethanol'''
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'''Sodium acetate'''<hr color='white' width='70%'>
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*'''Ethanol 70%'''
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'''Absolute ethanol'''<hr color='white' width='70%'>
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*'''ddH2O'''
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'''Ethanol 70%'''<hr color='white' width='70%'>
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*'''DNA sample'''
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'''ddH2O'''<hr color='white' width='70%'>
 +
'''DNA sample'''</font>
</td></tr></table><br><br><br><br>
</td></tr></table><br><br><br><br>
</td></tr></table>
</td></tr></table>

Revision as of 16:37, 22 September 2009

EthanolPVanimation.gif



Protocols

DNA precipitation with sodium acetate

(estimated time: 1 hour)

  • Add 1/10 DNA solution volume of sodium acetate 3 M
  • Add 2.5 DNA solution volume of absolute ethanol
  • Freeze at -80°C for 30 min
  • Centrifuge at 13000 rpm, 4°C for 20 min
  • Decant supernatant
  • Add 50 µl of 70% ethanol
  • Centrifuge at 13000 rpm, 4°C for 20 min
  • Remove all supernatant with a pipette
  • Air dry pellet until ethanol is totally removed
  • Elute with 5-10 µl of ddH2O




Sodium acetate
Absolute ethanol
Ethanol 70%
ddH2O

DNA sample





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