Team:USTC/Modeling/Model-2

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We employed an ODE model. We defined the input to be AHL<sub>out</sub> the and the output to be the synthesis rate of mature GFP.
We employed an ODE model. We defined the input to be AHL<sub>out</sub> the and the output to be the synthesis rate of mature GFP.
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To simplify the model, the (Recation S2-4), (Recation S2-5) and (Recation S2-4) were considered to get balance in a very shortly time, that is, the LuxR<sub>2</sub>, AHL<sub>in</sub>,LuxR<sub>2</sub>•AHl<sub>in</sub>, pLux/Tet, pLux/Tet•LuxR<sub>2</sub>•AHL<sub>2</sub> and pLux/Tet•LuxR<sub>2</sub> were constant in the system.
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[[Image:s-m-2-1.jpg]]
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To simplify the model, the (Recation S2-4), (Recation S2-5) and (Recation S2-4) were considered to be able to get balance in a very shortly time.
The values of 4.8E-3 for rmG, 4.8E-3 for a and 0.4 for pG were got from the F2620, and the value for d of 0.00368 based on the measurement of the OD.
The values of 4.8E-3 for rmG, 4.8E-3 for a and 0.4 for pG were got from the F2620, and the value for d of 0.00368 based on the measurement of the OD.
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[[Image:s-m-2-2.jpg]]

Revision as of 07:34, 21 October 2009

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Team:USTC/Modeling/Model-2

Contents


pConX2+LuxR+pLux/Tet+GFP

Parts: K176026 K176126 K176128 K176130

Model-2-2.jpg

Simplified Model-2

We employed an ODE model. We defined the input to be AHLout the and the output to be the synthesis rate of mature GFP.

S-m-2-1.jpg

To simplify the model, the (Recation S2-4), (Recation S2-5) and (Recation S2-4) were considered to be able to get balance in a very shortly time.

The values of 4.8E-3 for rmG, 4.8E-3 for a and 0.4 for pG were got from the F2620, and the value for d of 0.00368 based on the measurement of the OD.

S-m-2-2.jpg

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