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Team:USTC/Modeling/Model-2
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Team:USTC/Modeling/Model-2
Contents |
pConX2+LuxR+pLux/Tet+GFP
Parts: K176026 K176126 K176128 K176130
Simplified Model-2
We employed an ODE model. We defined the input to be AHLout the and the output to be the synthesis rate of mature GFP.
To simplify the model, the (Recation S2-4), (Recation S2-5) and (Recation S2-4) were considered to get balance in a very shortly time, that is, the LuxR2, AHLin,LuxR2•AHlin, pLux/Tet, pLux/Tet•LuxR2•AHL2 and pLux/Tet•LuxR2 were constant in the system.
The values of 4.8E-3 for rmG, 4.8E-3 for a and 0.4 for pG were got from the F2620, and the value for d of 0.00368 based on the measurement of the OD.
