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Team:USTC/Modeling/Model-5
From 2009.igem.org
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== '''Simplified Model-5''' == | == '''Simplified Model-5''' == | ||
| - | To get the datails of the model-processing, click [[Team:USTC/Modeling/Model-2|'''Simplified Model-2]] | + | To get the datails of the model-processing, click [[Team:USTC/Modeling/Model-2|'''Simplified Model-2''']] |
We employed an ODE model. We defined the input to be AHL<sub>out</sub> the and the output to be the synthesis rate of mature GFP. In this system,we assumed the concentration of the LuxR was constant. | We employed an ODE model. We defined the input to be AHL<sub>out</sub> the and the output to be the synthesis rate of mature GFP. In this system,we assumed the concentration of the LuxR was constant. | ||
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| - | For a particular concentration of AHL<sub>out</sub>, the value of parameter d could be estimated based on the measurement of the OD. | + | For a particular concentration of AHL<sub>out</sub>, the value of parameter d could be estimated based on the measurement of the OD. |
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| + | The values of C<sub>1</sub>, C<sub>2</sub>, C<sub>3</sub>, C<sub>4</sub> were same to be those of model-2, click [[Team:USTC/Modeling/Model-2|'''here''']] to the '''Simplified Model-2'''. | ||
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| + | The values of 4.8E-3 per sec for rmG, 4.8E-3 per sec for a and 0.4 per sec for pG were got from the experiment conducted by Barry Canton and Anna Labno in 2004, click [http://partsregistry.org/Part:BBa_F2620:Experience/Endy/Data_analysis here] to the details. | ||
[[Image:SM5-3.jpg|450px]] | [[Image:SM5-3.jpg|450px]] | ||
Useing the data from our experiments, we succeeded to estimate the values of . The simulation results as follows. | Useing the data from our experiments, we succeeded to estimate the values of . The simulation results as follows. | ||
Revision as of 23:47, 21 October 2009
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Team:USTC/Modeling/Model-5
Contents |
pConX1+LuxI+pConX2+LuxR+pLux/Tet+GFP
Parts:
Simplified Model-5
To get the datails of the model-processing, click Simplified Model-2
We employed an ODE model. We defined the input to be AHLout the and the output to be the synthesis rate of mature GFP. In this system,we assumed the concentration of the LuxR was constant.
To simplify the model, the (Recation S2-4), (Recation S2-5) and (Recation S2-6) were considered to be able to get balance in a very short time.
From above equations, we can the change of PoPS respond to the AHLin.
For a particular concentration of AHLout, the value of parameter d could be estimated based on the measurement of the OD.
The values of C1, C2, C3, C4 were same to be those of model-2, click here to the Simplified Model-2.
The values of 4.8E-3 per sec for rmG, 4.8E-3 per sec for a and 0.4 per sec for pG were got from the experiment conducted by Barry Canton and Anna Labno in 2004, click here to the details.
Useing the data from our experiments, we succeeded to estimate the values of . The simulation results as follows.
