Team:Uppsala-Sweden/Butanol

From 2009.igem.org

(Difference between revisions)

Revision as of 19:33, 26 July 2009

The Butanol Project

Our goal is to introduce isobutanol producing capability into Synechocystis sp. PCC 6803 using the biobrick system.

How to

Cyanobacteria have the capability to harvest the energy from the sun and convert it into other forms of energy. The natural way for these organisms is to store it as sugars or other carbohydrates in a way similar to plants. By introducing a casette of genes for isobutanol production, we could get a cyanobacteria to start producing isobutanol. The precursor for isobutanol production is pyruvate which is further processed to amino acid precursors. We want to hijack the pathway by introducing two genes which use these precursors to produce isobtuanol from them.

The Construct

Here is the preliminary construct for Synechocystis 6803.

@@ Line 4: / Line 4: @@
 Our goal is to introduce isobutanol producing capability into Synechocystis sp. PCC 6803 using the biobrick system.
-==How to ==
+==How to==
-Cyano bacteria have the capability to harvest the energy from the sun and convert it into other forms of energy. The natural way for these organisms is to store it as sugars or other carbohydrates in a way similar to plants.
+Cyanobacteria have the capability to harvest the energy from the sun and convert it into other forms of energy. The natural way for these organisms is to store it as sugars or other carbohydrates in a way similar to plants. By introducing a casette of genes for isobutanol production, we could get a cyanobacteria to start producing isobutanol. The precursor for isobutanol production is pyruvate which is further processed to amino acid precursors. We want to hijack the pathway by introducing two genes which use these precursors to produce isobtuanol from them.
-By introducing a casette of genes for isobutanol production, we could get a cyano bacteria to start producing isobutanol. The precursor for isobutanol production is pyruvate which is further processed to amino acid precursors. We want to hijack the pathway by introducing two genes which use this precursor to produce isobtuanol from them.
 ==The Construct==