Team:Valencia/Project

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== '''Project description''' ==
== '''Project description''' ==
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<span style="color:black; align:justify; font-size:11pt; font-family:Verdana"> The <b>iGEM Valencia Lighting Cell Display</b> (<b>iLCD</b>) is our project for the present iGEM competition. We are developing <b>a “bio-screen” of voltage-activated cells</b>, where every “cellular pixel” produces light. </span>
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The <b>iGEM Valencia Lighting Cell Display</b> (<b>iLCD</b>) is our project for the present iGEM competition. We are developing '''BioElectronics''', a combination of Electronics and Biology. We hereby prove that cell behaviour is controlled by electrical pulses. In order to demonstrate this, we are making <b>a “bio-screen” of voltage-activated cells</b>, where every “cellular pixel” produces light. '''It is just like a bacterial photographic system, but it's digital'''. Within seconds, instead of hours, you can get an image formed of living cells.
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<span style="color:black; align:justify; font-size:11pt; font-family: Verdana">It is known that for instance <b>neurons, cardiomyocites or muscle cells</b> are able to sense and respond to electrical signals. These cells use a common second messenger system, calcium ion, which promotes a defined response when an electrical pulse is supplied to them. Nevertheless, these cultures present several disadvantadges in order to make use of them from the technological point of view:</span>
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It is known that for instance <b>neurons, cardiomyocites or muscle cells</b> are able to sense and respond to electrical signals. These cells use a common second messenger system, calcium ion, which promotes a defined response when an electrical pulse is supplied to them. Nevertheless, these cultures present several disadvantages in order to use them from the technological point of view:  
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<span style="color:black; align:justify; font-size:11pt; font-family: Verdana">- Get easily contaminated.</span>
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* Get easily contaminated.
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<span style="color:black; align:justify; font-size:11pt; font-family: Verdana">- Genetic manipulation is complicated and expensive.</span>
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* Genetic manipulation is complicated and expensive.
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<span style="color:black; align:justify; font-size:11pt; font-family: Verdana">- To be very sensible to external conditions. </span>
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* To be very sensible to external conditions.  
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<span style="color:black; align:justify; font-size:11pt; font-family: Verdana">Valencia team uses this sensibility of calcium channel to  electricity to <b>produce yeast luminiscence as a response to electrical estimulous</b>. This project constitutes the '''FIRST TIME in which the electrical response of <i>Saccharomyces</i> and its potential applications are going to be tested''' building the first '''LEC''' (Light Emitting Cell). The obtained device will be used to build the first''' iLCD''' in history.</span>
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Valencia Team uses this electricity sensibility of calcium channel to <b>produce yeast luminescence as a response to electrical estimulous</b>. This project constitutes the '''FIRST TIME in which the electrical response of <i>Saccharomyces</i> and its potential applications have been tested''' building the first '''LEC''' (Light Emitting Cell). The obtained device will be used to build the first '''iLCD''' in history.
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<span style="color:black; align:justify; font-size:11pt; font-family: Verdana">Therefore, the project is divided in several stages from the fabrication of the first '''LEC''' up to the cooperative integration of various LECs in the first '''iLCD'''. The global scheme of the project is summarized in the scheme of the figure:</span>
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Therefore, the project is divided in several stages from the fabrication of the first '''LEC''' up to the cooperative integration of various LECs in the first '''iLCD'''. The global scheme of the project is summarized in the following scheme:
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Three main parts can be appreciated:
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<span style="color:black; align:justify; font-size:11pt; font-family: Verdana">Where three main parts can be appreciated</span>
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* LEC Construction.
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<span style="color:black; align:justify; font-size:11pt; font-family: Verdana">* LEC Construction</span>
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* LEC Characterization.
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<span style="color:black; align:justify; font-size:11pt; font-family: Verdana">* LEC Characterization</span>
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* iLCD: LEC Integration Device.
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<span style="color:black; align:justify; font-size:11pt; font-family: Verdana">* LEC Integration Device</span>
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<span style="color:black; align:justify; font-size:11pt; font-family: Verdana">The main advantages of using electrical signals instead of chemical stimulation, as in the Coliroid project (Levskaya et al, <i>Synthetic biology: Engineering Escherichia coli to see light</i>. <b>Nature</b> 438, 441-442),  are reversibility and high frequency: the system can go back to the resting state and it will take <b>milliseconds to refresh an image, actually showing animated pictures!</b>.</span>
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The main advantages of using electrical signals instead of chemical stimulation, as in the Coliroid project (Levskaya et al, <i>Synthetic biology: Engineering Escherichia coli to see light</i>. <b>Nature</b> 438, 441-442),  are reversibility and high frequency: the system goes back to the resting state and it take <b>seconds (down to 12 seconds) to refresh an image, actually showing animated pictures!</b>. For that reason, we chose the calcium signaling because it is the fastest known modality of signaling in biology, and will allow for a fast refreshing rate of the screen.
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<span style="color:black; align:justify; font-size:11pt; font-family: Verdana"><b>iLCD will be a major advance in Synthetic Biology, opening the field of Green Electronics, integrating electrical signals with cell behaviours</b>. This will reduce the response time of the cells to the activation signal by up to two orders of magnitude, as well as foster the combination of Electronics and Biology.</span>
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<b>iLCD will be a major advance in Synthetic Biology, opening the field of BioElectronics, integrating electrical signals with cell behaviours</b>. This will reduce the response time of the cells to the activation signal by up to two orders of magnitude, as well as foster the combination of Electronics and Biology. Thus, our engineered yeast are a state-of-art bioelectronic device.
   
   
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<span style="color:red; text-align:center; font-size:12pt; font-family: Verdana"><b>See the Notebook and News and stay tuned for news and progresses!!!</b></span>
 
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Latest revision as of 03:22, 22 October 2009


Project description


The iGEM Valencia Lighting Cell Display (iLCD) is our project for the present iGEM competition. We are developing BioElectronics, a combination of Electronics and Biology. We hereby prove that cell behaviour is controlled by electrical pulses. In order to demonstrate this, we are making a “bio-screen” of voltage-activated cells, where every “cellular pixel” produces light. It is just like a bacterial photographic system, but it's digital. Within seconds, instead of hours, you can get an image formed of living cells.

It is known that for instance neurons, cardiomyocites or muscle cells are able to sense and respond to electrical signals. These cells use a common second messenger system, calcium ion, which promotes a defined response when an electrical pulse is supplied to them. Nevertheless, these cultures present several disadvantages in order to use them from the technological point of view:

  • Get easily contaminated.
  • Genetic manipulation is complicated and expensive.
  • To be very sensible to external conditions.

Valencia Team uses this electricity sensibility of calcium channel to produce yeast luminescence as a response to electrical estimulous. This project constitutes the FIRST TIME in which the electrical response of Saccharomyces and its potential applications have been tested building the first LEC (Light Emitting Cell). The obtained device will be used to build the first iLCD in history.

Therefore, the project is divided in several stages from the fabrication of the first LEC up to the cooperative integration of various LECs in the first iLCD. The global scheme of the project is summarized in the following scheme:


Three main parts can be appreciated:

  • LEC Construction.
  • LEC Characterization.
  • iLCD: LEC Integration Device.

The main advantages of using electrical signals instead of chemical stimulation, as in the Coliroid project (Levskaya et al, Synthetic biology: Engineering Escherichia coli to see light. Nature 438, 441-442), are reversibility and high frequency: the system goes back to the resting state and it take seconds (down to 12 seconds) to refresh an image, actually showing animated pictures!. For that reason, we chose the calcium signaling because it is the fastest known modality of signaling in biology, and will allow for a fast refreshing rate of the screen.

iLCD will be a major advance in Synthetic Biology, opening the field of BioElectronics, integrating electrical signals with cell behaviours. This will reduce the response time of the cells to the activation signal by up to two orders of magnitude, as well as foster the combination of Electronics and Biology. Thus, our engineered yeast are a state-of-art bioelectronic device.