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| - | {{Template:Valencia09iGEM}} | + | {{Template:Valencia09iGEM2}} |
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| - | <div align="justify" style="position:relative; top:-5px; left:70px; width:500px"> | + | <table cellpadding="5" cellspacing="5" overflow="hidden" align="center"> |
| - | == '''Yeast Team''' ==
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| - | <span style="color:black; align:justify; font-size:10pt; font-family: Verdana">
| + | <html><a href="https://2009.igem.org/Team:Valencia/WetLab/YeastTeam" target="_top"> <img src="https://static.igem.org/mediawiki/2009/e/e8/Yeastteam.jpg" width="300" height="230"></a></html> |
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| - | An important part of our project is to work with '''yeasts''' in order to create each and everyone of the cell-pixels of our bioscreen. We will use some aequorin-transformed yeast strains.
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| - | '''Aequorin''' is a luminiscent protein isolated from luminescent jellyfish and many other marine organisms that uses '''coelenterazine''' as its cofactor to produce light whenever Ca<SUP>2+</SUP> is bound to it. Using a '''chemical input''' (KOH, alkali shock) we will open '''Ca<SUP>2+</SUP> channels''' in the yeasts' membrane (Viladevall L, et al. J Biol Chem. (2004) 279 43614–43624), Ca<SUP>2+</SUP> will enter into the cells and we will get '''light''' as an '''output'''.
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| - | Later we will try to reproduce the same response with an '''electrical stimulus''', using some hardware built by ourselves.
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