Team:Virginia/4 August 2009

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(New page: {{Template:VirginiaNotebook}} ==Today== *Miniprep of ABP / ABP+HA in vectors **Conc: *Digest of above (E,P) **Gel: *New PCR to HA to ABP again *Temps:)
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==Today==
==Today==
*Miniprep of ABP / ABP+HA in vectors
*Miniprep of ABP / ABP+HA in vectors
-
**Conc:
+
**Conc: 17.8ng/ul and 33.3ng/ul, respectively
*Digest of above (E,P)
*Digest of above (E,P)
**Gel:
**Gel:
*New PCR to HA to ABP again
*New PCR to HA to ABP again
-
*Temps:
+
**Temps: 54C for 6 cycles for overlap part to bind, then 60C for 24 cycles (calculated salt-adjusted temp for long primer is 87C, shorter one is 63C)

Latest revision as of 18:56, 4 August 2009

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Today

  • Miniprep of ABP / ABP+HA in vectors
    • Conc: 17.8ng/ul and 33.3ng/ul, respectively
  • Digest of above (E,P)
    • Gel:
  • New PCR to HA to ABP again
    • Temps: 54C for 6 cycles for overlap part to bind, then 60C for 24 cycles (calculated salt-adjusted temp for long primer is 87C, shorter one is 63C)