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Team:Virginia/4 August 2009
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==Today== | ==Today== | ||
*Miniprep of ABP / ABP+HA in vectors | *Miniprep of ABP / ABP+HA in vectors | ||
| - | **Conc: | + | **Conc: 17.8ng/ul and 33.3ng/ul, respectively |
*Digest of above (E,P) | *Digest of above (E,P) | ||
**Gel: | **Gel: | ||
*New PCR to HA to ABP again | *New PCR to HA to ABP again | ||
| - | **Temps: | + | **Temps: 54C for 6 cycles for overlap part to bind, then 60C for 24 cycles (calculated salt-adjusted temp for long primer is 87C, shorter one is 63C) |
Latest revision as of 18:56, 4 August 2009
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Today
- Miniprep of ABP / ABP+HA in vectors
- Conc: 17.8ng/ul and 33.3ng/ul, respectively
- Digest of above (E,P)
- Gel:
- New PCR to HA to ABP again
- Temps: 54C for 6 cycles for overlap part to bind, then 60C for 24 cycles (calculated salt-adjusted temp for long primer is 87C, shorter one is 63C)
