Team:Virginia Commonwealth/Internal/Stocks

From 2009.igem.org

(Difference between revisions)
(Box 1 (orange bottom))
(Long-term glycerol stocks)
 
(13 intermediate revisions not shown)
Line 12: Line 12:
===Seed cells (Box C-??)===
===Seed cells (Box C-??)===
*Insert chart here
*Insert chart here
 +
 +
===Box C11 (Afton and Maria)===
 +
{| Border="1" align="center"
 +
|1: J23100 || 2: J23101 || 3: J23102 || 4: J23103 || 5: J23104 || 6:  J23105 || 7: J23106 || 8: J23107 || 9:
 +
|-
 +
|10: || 11: || 12: || 13: || 14: || 15: || 16: || 17: || 18:
 +
|-
 +
|19: || 20: J23110|| 21: || 22: || 23: || 24: || 25: || 26: || 27:
 +
|-
 +
|28: J23106 || 29: J23106 || 30: J23106 || 31: || 32: || 33: || 34: || 35: || 36:
 +
|-
 +
|37: J06702 || 38: J06702 || 39: J06702 || 40: J06702 || 41: J06702 || 42: || 43: || 44: || 45:
 +
|-
 +
|46: E0240 || 47: E0240 || 48: E0240 || 49: || 50: || 51: || 52: || 53: || 54:
 +
|-
 +
|55: || 56: || 57: || 58: || 59: || 60: || 61: || 62: || 63:
 +
|-
 +
|64: || 65: || 66: || 67: || 68: || 69: || 70: || 71: || 72:
 +
|-
 +
|73: || 74: || 75: || 76: || 77: || 78: || 79: || 80: || 81:
 +
|}
==Short-term plasmid stocks==
==Short-term plasmid stocks==
-
-20 degrees C
+
====-20 degrees C====
===Box 1 (Kevin and Adam)===
===Box 1 (Kevin and Adam)===
-
*Insert chart here
 
-
===Box 2 (Afton and Maria)===
+
{| Border="1" align="center"
-
*Insert chart here
+
|1:BBa_J23101 || 2:BBa_J23119 || 3:BBa_E0840 || 4:BBa_J23119 || 5:BBa_J23101 || 6:BBa_E0840 || 7:|| 8:|| 9:
 +
|-
 +
|10: || 11: || 12: || 13: || 14: || 15: || 16: || 17: || 18:
 +
|-
 +
|19: || 20: || 21: || 22: || 23: || 24: || 25: || 26: || 27:
 +
|-
 +
|28: || 29: || 30: || 31: || 32: || 33: || 34: || 35: || 36:
 +
|-
 +
|37: || 38: || 39: || 40: || 41: || 42: || 43: || 44: || 45:
 +
|-
 +
|46: || 47: || 48: || 49: || 50: || 51: || 52: || 53: || 54:
 +
|-
 +
|55: || 56: || 57: || 58: || 59: || 60: || 61: || 62: || 63:
 +
|-
 +
|64: || 65: || 66: || 67: || 68: || 69: || 70: || 71: || 72:
 +
|-
 +
|73: || 74: || 75: || 76: || 77: || 78: || 79: || 80: || 81:
 +
|}
 +
 
 +
===Box 2 (Afton and Maria BioBrick Stocks)===
 +
*Unless Specified all have been mini-prepped. REMEMBER to find out the concentration before running a digest.
 +
{| Border="1" align="center"
 +
|1:BBa_J23102 || 2:BBa_J23104 || 3:BBa_J23100 || 4:BBa_J23110 || 5:BBa_R0040 || 6:BBa_J23116 || 7:BBa_J23105 || 8:BBa_J23113 || 9:BBa_J23107
 +
|-
 +
|10:BBa_J23103 || 11:BBa_R0011 || 12:BBa_J23101 || 13:BBa_E0240 || 14:BBa_E0240 || 15:BBa_E0240 || 16:BBa_E0240 || 17:BBa_E0240 || 18:BBa_J23102
 +
|-
 +
|19:pSB1C3 || 20:pSB4C5 || 21:BBa_J13002 || 22:Ba_R0040 || 23:BBa_E1010 || 24:BBa_I13521 || 25:BBa_J04450 || 26:BBa_E0040 || 27:pSB4C5
 +
|-
 +
|28:pSB1C3 || 29:BBa_J04450 || 30: || 31: || 32: || 33: || 34: || 35: || 36:
 +
|-
 +
|37: || 38: || 39: || 40: || 41: || 42: || 43: || 44: || 45:
 +
|-
 +
|46: || 47: || 48: || 49: || 50: || 51: || 52: || 53: || 54:
 +
|-
 +
|55: || 56: || 57: || 58: || 59: || 60: || 61: || 62: || 63:
 +
|-
 +
|64: || 65: || 66: || 67: || 68: || 69: || 70: || 71: || 72:
 +
|-
 +
|73: || 74: || 75: || 76: || 77: || 78: || 79: || 80: || 81:
 +
|}
===Box 3 (Craig and Clay)===
===Box 3 (Craig and Clay)===
-
*Insert chart here
+
{| Border="1" align="center"
 +
|1: || 2: || 3: || 4: || 5: || 6: || 7:|| 8:|| 9:
 +
|-
 +
|10: || 11: || 12: || 13: || 14: || 15: || 16: || 17: || 18:
 +
|-
 +
|19: || 20: || 21: || 22: || 23: || 24: || 25: || 26: || 27:
 +
|-
 +
|28: || 29: || 30: || 31: || 32: || 33: || 34: || 35: || 36:
 +
|-
 +
|37: || 38: || 39: || 40: || 41: || 42: || 43: || 44: || 45:
 +
|-
 +
|46: || 47: || 48: || 49: || 50: || 51: || 52: || 53: || 54:
 +
|-
 +
|55: || 56: || 57: || 58: || 59: || 60: || 61: || 62: || 63:
 +
|-
 +
|64: || 65: || 66: || 67: || 68: || 69: || 70: || 71: || 72:
 +
|-
 +
|73: || 74: || 75: || 76: || 77: || 78: || 79: || 80: || 81:
 +
|}
 +
 
 +
===BioBrick DNA Distribution===
 +
 
 +
{|Border="1" align="center"
 +
!_!!A!!B!!C!!D!!E
 +
|-
 +
!1||BBa_J23104||BBa_J23113||BBa_E1010||BBa_I20260||BBa_J23116
 +
|-
 +
!2||BBa_J23119||pSB1AK3||BBa_Q04511||BBa_E0840||BBa_I13521
 +
|-
 +
!3||BBa_J23110||BBa_J23106||BBa_J06702||BBa_J23103||BBa_J23101
 +
|-
 +
!4||pSB4C5||BBa_R0011||pSB1C3||BBa_J23100||BBa_J23107
 +
|-
 +
!5||BBa_J23102||BBa_J23105||BBa_J13002||BBa_E0240||BBa_R0040
 +
|-
 +
!6||pSB3K3 w/p1010||pSB3K3|| || ||
 +
|-
 +
!7|| || || || ||
 +
|-
 +
!8|| || || || ||
 +
|-
 +
!9|| || || || ||
 +
|}

Latest revision as of 16:02, 13 July 2009

Contents

Long-term glycerol stocks

-80 degrees C

Transformed cells (Box C-??)

  • Insert chart here

Competent cells (Box C-??)

  • Insert chart here

Seed cells (Box C-??)

  • Insert chart here

Box C11 (Afton and Maria)

1: J23100 2: J23101 3: J23102 4: J23103 5: J23104 6: J23105 7: J23106 8: J23107 9:
10: 11: 12: 13: 14: 15: 16: 17: 18:
19: 20: J23110 21: 22: 23: 24: 25: 26: 27:
28: J23106 29: J23106 30: J23106 31: 32: 33: 34: 35: 36:
37: J06702 38: J06702 39: J06702 40: J06702 41: J06702 42: 43: 44: 45:
46: E0240 47: E0240 48: E0240 49: 50: 51: 52: 53: 54:
55: 56: 57: 58: 59: 60: 61: 62: 63:
64: 65: 66: 67: 68: 69: 70: 71: 72:
73: 74: 75: 76: 77: 78: 79: 80: 81:

Short-term plasmid stocks

-20 degrees C

Box 1 (Kevin and Adam)

1:BBa_J23101 2:BBa_J23119 3:BBa_E0840 4:BBa_J23119 5:BBa_J23101 6:BBa_E0840 7: 8: 9:
10: 11: 12: 13: 14: 15: 16: 17: 18:
19: 20: 21: 22: 23: 24: 25: 26: 27:
28: 29: 30: 31: 32: 33: 34: 35: 36:
37: 38: 39: 40: 41: 42: 43: 44: 45:
46: 47: 48: 49: 50: 51: 52: 53: 54:
55: 56: 57: 58: 59: 60: 61: 62: 63:
64: 65: 66: 67: 68: 69: 70: 71: 72:
73: 74: 75: 76: 77: 78: 79: 80: 81:

Box 2 (Afton and Maria BioBrick Stocks)

  • Unless Specified all have been mini-prepped. REMEMBER to find out the concentration before running a digest.
1:BBa_J23102 2:BBa_J23104 3:BBa_J23100 4:BBa_J23110 5:BBa_R0040 6:BBa_J23116 7:BBa_J23105 8:BBa_J23113 9:BBa_J23107
10:BBa_J23103 11:BBa_R0011 12:BBa_J23101 13:BBa_E0240 14:BBa_E0240 15:BBa_E0240 16:BBa_E0240 17:BBa_E0240 18:BBa_J23102
19:pSB1C3 20:pSB4C5 21:BBa_J13002 22:Ba_R0040 23:BBa_E1010 24:BBa_I13521 25:BBa_J04450 26:BBa_E0040 27:pSB4C5
28:pSB1C3 29:BBa_J04450 30: 31: 32: 33: 34: 35: 36:
37: 38: 39: 40: 41: 42: 43: 44: 45:
46: 47: 48: 49: 50: 51: 52: 53: 54:
55: 56: 57: 58: 59: 60: 61: 62: 63:
64: 65: 66: 67: 68: 69: 70: 71: 72:
73: 74: 75: 76: 77: 78: 79: 80: 81:

Box 3 (Craig and Clay)

1: 2: 3: 4: 5: 6: 7: 8: 9:
10: 11: 12: 13: 14: 15: 16: 17: 18:
19: 20: 21: 22: 23: 24: 25: 26: 27:
28: 29: 30: 31: 32: 33: 34: 35: 36:
37: 38: 39: 40: 41: 42: 43: 44: 45:
46: 47: 48: 49: 50: 51: 52: 53: 54:
55: 56: 57: 58: 59: 60: 61: 62: 63:
64: 65: 66: 67: 68: 69: 70: 71: 72:
73: 74: 75: 76: 77: 78: 79: 80: 81:

BioBrick DNA Distribution

_ABCDE
1BBa_J23104BBa_J23113BBa_E1010BBa_I20260BBa_J23116
2BBa_J23119pSB1AK3BBa_Q04511BBa_E0840BBa_I13521
3BBa_J23110BBa_J23106BBa_J06702BBa_J23103BBa_J23101
4pSB4C5BBa_R0011pSB1C3BBa_J23100BBa_J23107
5BBa_J23102BBa_J23105BBa_J13002BBa_E0240BBa_R0040
6pSB3K3 w/p1010pSB3K3
7
8
9