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Team:Washington/Notebook
From 2009.igem.org
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==== Project Time Line ==== | ==== Project Time Line ==== | ||
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*Winter Quarter | *Winter Quarter | ||
**Introduction to iGEM | **Introduction to iGEM | ||
**Synthetic Biology Seminar | **Synthetic Biology Seminar | ||
**Plan for project ideas | **Plan for project ideas | ||
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*Spring Quarter | *Spring Quarter | ||
**Narrow down potential projects | **Narrow down potential projects | ||
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**Obtained Funding | **Obtained Funding | ||
**Stock Lab | **Stock Lab | ||
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*June | *June | ||
**Sequence genes | **Sequence genes | ||
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**Test target proteins for solubility and expression | **Test target proteins for solubility and expression | ||
**Start assembly of secretion genes | **Start assembly of secretion genes | ||
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*July | *July | ||
**Develop and perform assays for testing legacy surface display bio-bricks | **Develop and perform assays for testing legacy surface display bio-bricks | ||
**Transfer prtDEF contig from secretion system into low copy plasmid | **Transfer prtDEF contig from secretion system into low copy plasmid | ||
**Test target proteins for functionality | **Test target proteins for functionality | ||
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*August | *August | ||
**Finish assembly of secretion system | **Finish assembly of secretion system | ||
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**Test for secretion of target protein | **Test for secretion of target protein | ||
**Start design of new display construct | **Start design of new display construct | ||
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*September | *September | ||
**Switch secretion system into new cell line, make cells competent | **Switch secretion system into new cell line, make cells competent | ||
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**Start t-shirt design | **Start t-shirt design | ||
**Insert streptavidin into new display vector | **Insert streptavidin into new display vector | ||
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*October | *October | ||
**Fine tune secretion assay, adjust controls | **Fine tune secretion assay, adjust controls | ||
Revision as of 05:02, 15 October 2009
Protocols
Supernatant Protein Purification, 50mL
Supernatant Protein Purification, 2mL
Gene Assembly using the NheI and PstI sites
Gene Assembly using the NheI site
Project Time Line
- Winter Quarter
- Introduction to iGEM
- Synthetic Biology Seminar
- Plan for project ideas
- Spring Quarter
- Narrow down potential projects
- Choose Project
- Order oligos and start synthesizing genes
- Obtained Funding
- Stock Lab
- June
- Sequence genes
- Preliminary binding assays for biotinylated fluorophore
- Introduction to Fold-it as a tool for protein design
- Test target proteins for solubility and expression
- Start assembly of secretion genes
- July
- Develop and perform assays for testing legacy surface display bio-bricks
- Transfer prtDEF contig from secretion system into low copy plasmid
- Test target proteins for functionality
- August
- Finish assembly of secretion system
- Start cell lines containing various forms of secretion system, make competent
- Transform competent cells containing secretion system with target vector
- Test for secretion of target protein
- Start design of new display construct
- September
- Switch secretion system into new cell line, make cells competent
- Transform with target vector
- Test for secretion
- Start Presentation
- Start t-shirt design
- Insert streptavidin into new display vector
- October
- Fine tune secretion assay, adjust controls
- Finalize characterization of legacy parts
- Practice presentation
- Characterize target bio-bricks
- Prepare for Jamboree
