Team:Wisconsin-Madison/Cloning Protocols

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Overview of Cloning Protocol Solid Culture Liquid Culture Plasmid Isolation Restriction Enzyme Mapping for Verification PCR to add BioBrick Restriction Cut Sites (Primer Design) PCR Plasmid Cleanup Concentration Verification Digestion Digestion Clean Up Concentration Verification Ligation Transformation Colony PCR Screening Procedures for these can be found in Protocol Section Back to Protocols