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Template:Team:KULeuven/12 August 2009/VanillinReceptor
From 2009.igem.org
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* B,G and R were stored for further procedure. A failed again because there was no growth visible. This second time there was no problem with the competent cells because we used a commercial product and not self made. We assumed there was a problem with adding the polyA tales to the PCR product because we did a new gelelectroforesis and there were no errors with the PCR product. | * B,G and R were stored for further procedure. A failed again because there was no growth visible. This second time there was no problem with the competent cells because we used a commercial product and not self made. We assumed there was a problem with adding the polyA tales to the PCR product because we did a new gelelectroforesis and there were no errors with the PCR product. | ||
*We did a new PCR on the bacteria with the inserted plasmids for B,G and R to check if the gene certainly was inserted correct. | *We did a new PCR on the bacteria with the inserted plasmids for B,G and R to check if the gene certainly was inserted correct. | ||
| - | *PCR was performed for W,X,Y and Z. | + | *PCR was performed for W,X,Y and Z to isolate the genes. |
Revision as of 12:40, 25 August 2009
- B,G and R were stored for further procedure. A failed again because there was no growth visible. This second time there was no problem with the competent cells because we used a commercial product and not self made. We assumed there was a problem with adding the polyA tales to the PCR product because we did a new gelelectroforesis and there were no errors with the PCR product.
- We did a new PCR on the bacteria with the inserted plasmids for B,G and R to check if the gene certainly was inserted correct.
- PCR was performed for W,X,Y and Z to isolate the genes.
