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Template:Team:KULeuven/1 September 2009/BlueLightReceptor
From 2009.igem.org
(Difference between revisions)
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#** 4 agar plates with {{kulpart|BBa_E0240}} = 2 for irradiation and 2 for control. | #** 4 agar plates with {{kulpart|BBa_E0240}} = 2 for irradiation and 2 for control. | ||
#** 4 liquid cultures with {{kulpart|BBa_E0240}} = 2 for irradiation and 2 for control. | #** 4 liquid cultures with {{kulpart|BBa_E0240}} = 2 for irradiation and 2 for control. | ||
| - | #* | + | #* there are two groups, each with 8 cultures. |
| - | #** 2 agar plates and 2 liquid cultures with LigA and 2 agar plates and 2 liquid cultures with {{kulpart|BBa_E0240}} were put in 16°C for one hour. after an hour half of them were irradiated with blue light for an hour while the other have was wrapped in alluminium foil as a control. | + | #** shift one: 2 agar plates and 2 liquid cultures with LigA and 2 agar plates and 2 liquid cultures with {{kulpart|BBa_E0240}} were put in 16°C for one hour. after an hour half of them were irradiated with blue light for an hour while the other have was wrapped in alluminium foil as a control. afterwards they are put in the 37°C incubator for some time |
| + | #** shift two: they were put in the 37°C for some hours so that the cultures can grow. | ||
Revision as of 10:03, 1 September 2009
- experimental set up for irradiation of LigA ( + )
- enting colonies from the motherplate:
- 4 agar plates with LigA = 2 for irradiation and 2 for control.
- 4 liquid cultures with LigA = 2 for irradiation and 2 for control.
- 4 agar plates with = 2 for irradiation and 2 for control.
- 4 liquid cultures with = 2 for irradiation and 2 for control.
- there are two groups, each with 8 cultures.
- shift one: 2 agar plates and 2 liquid cultures with LigA and 2 agar plates and 2 liquid cultures with were put in 16°C for one hour. after an hour half of them were irradiated with blue light for an hour while the other have was wrapped in alluminium foil as a control. afterwards they are put in the 37°C incubator for some time
- shift two: they were put in the 37°C for some hours so that the cultures can grow.
- enting colonies from the motherplate:
