"
Template:Team:KULeuven/29 July 2009/BlueLightReceptor
From 2009.igem.org
(Difference between revisions)
(New page: GFP ({{kulpart|BBa_E0240}} *miniprepped and nanodropped **concentration: 85,6ng/μl **260/280: 1,87 * a restriction digest was performed to cut the plasmid with EcoRI and XbaI **a mixture...) |
|||
| Line 3: | Line 3: | ||
**concentration: 85,6ng/μl | **concentration: 85,6ng/μl | ||
**260/280: 1,87 | **260/280: 1,87 | ||
| - | * a restriction digest was performed to cut the plasmid with EcoRI and XbaI | + | *a restriction digest was performed to cut the plasmid with EcoRI and XbaI |
**a mixture of 20μl was made: | **a mixture of 20μl was made: | ||
6μl DNA | 6μl DNA | ||
Revision as of 11:26, 29 July 2009
GFP (
- miniprepped and nanodropped
- concentration: 85,6ng/μl
- 260/280: 1,87
- a restriction digest was performed to cut the plasmid with EcoRI and XbaI
- a mixture of 20μl was made:
6μl DNA 2μl buffer H 1μl EcoRI and 1μl XbaI 10μl AD
- the mixture was incubated for at least an hour at 37°C
