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Template:Team:KULeuven/29 July 2009/BlueLightReceptor
From 2009.igem.org
(Difference between revisions)
| Line 4: | Line 4: | ||
**260/280: 1,87 | **260/280: 1,87 | ||
*a restriction digest was performed to cut the plasmid with EcoRI and XbaI | *a restriction digest was performed to cut the plasmid with EcoRI and XbaI | ||
| - | **a mixture of 20μl was made: | + | **a mixture of 20μl was made:6μl DNA, 2μl bufferH, 1μl EcoRI and 1μl XbaI, 10μl AD |
| - | 6μl DNA | + | |
| - | 2μl | + | |
| - | 1μl EcoRI and 1μl XbaI | + | |
| - | 10μl AD | + | |
**the mixture was incubated for at least an hour at 37°C | **the mixture was incubated for at least an hour at 37°C | ||
Revision as of 11:28, 29 July 2009
GFP (
- miniprepped and nanodropped
- concentration: 85,6ng/μl
- 260/280: 1,87
- a restriction digest was performed to cut the plasmid with EcoRI and XbaI
- a mixture of 20μl was made:6μl DNA, 2μl bufferH, 1μl EcoRI and 1μl XbaI, 10μl AD
- the mixture was incubated for at least an hour at 37°C
