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Template:Team:KULeuven/29 July 2009/BlueLightReceptor
From 2009.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | GFP ({{kulpart|BBa_E0240}} | + | GFP ({{kulpart|BBa_E0240}}) |
*miniprepped and nanodropped | *miniprepped and nanodropped | ||
**concentration: 85,6ng/μl | **concentration: 85,6ng/μl | ||
Revision as of 11:28, 29 July 2009
GFP ()
- miniprepped and nanodropped
- concentration: 85,6ng/μl
- 260/280: 1,87
- a restriction digest was performed to cut the plasmid with EcoRI and XbaI
- a mixture of 20μl was made:6μl DNA, 2μl bufferH, 1μl EcoRI and 1μl XbaI, 10μl AD
- the mixture was incubated for at least an hour at 37°C
