Template:Team:KULeuven/29 July 2009/BlueLightReceptor

(Difference between revisions)

Revision as of 13:33, 29 July 2009

GFP ()

miniprepped and nanodropped
- concentration: 85,6ng/μl
- 260/280: 1,87
a restriction digest was performed to cut the plasmid with EcoRI and XbaI
- a mixture of 20μl was made:6μl DNA, 2μl bufferH, 1μl EcoRI and 1μl XbaI, 10μl AD
- the mixture was incubated for at least an hour at 37°C

BLR promoter region The PCR product that was purified friday (24/07) is digested with EcoRI and partially digested with SpeI

digestion with EcoRI
- following mixture was made (x6): 5μl DNA, 2μl bufferH, 1μl EcoRI, 12μl MilliQ
- incubated for 1h at 37°C
partial digestion with SpeI
- dilution of the enzymes:
  - AD/b: 225μl MQ + 25μl bufferH
  - 1/100: 1μl SpeI + 99μl AD/b
  - 1/200: 50μl 1/100 + 50μl AD/b
  - 1/500: 20μl 1/100 + 80μl AD/b
- making following mixture:

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