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Template:Team:KULeuven/2 September 2009/BlueLightReceptor
From 2009.igem.org
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# after nanodropping, {{kulpart|BBa_J23101}} was restricted with SpeI and PstI, while {{kulpart|BBa_E0240}} was cut with PstI and XbaI. | # after nanodropping, {{kulpart|BBa_J23101}} was restricted with SpeI and PstI, while {{kulpart|BBa_E0240}} was cut with PstI and XbaI. | ||
| - | # a gel electroforesis and extraction was performed | + | # a gel electroforesis and extraction was performed. this showed a 800 bp signal at the lanes with {{kulpart|BBa_J23101}}, which is not expected. |
# DB3.1, J23101 and LigA were ented in liquid culture and grown overnight to make glycerol stocks and competent cells | # DB3.1, J23101 and LigA were ented in liquid culture and grown overnight to make glycerol stocks and competent cells | ||
Revision as of 14:47, 2 September 2009
- FACS measurements:
- the cultures from shift 2 had similar results as shift one. however, the GFP signal measured from the LigA construct was stronger. thus, cells definitely need to grow before undergoing irradiation.
- the dillutions and the shift one cultures put overnight did not show any significant results.
- was miniprepped and nanodropped
| Part | concentration (ng/μl) | 260/280 λ | |
|---|---|---|---|
| (A) | 238,9 | 1,95 | |
| (B) | 248,8 | 1,91 |
- after nanodropping, was restricted with SpeI and PstI, while was cut with PstI and XbaI.
- a gel electroforesis and extraction was performed. this showed a 800 bp signal at the lanes with , which is not expected.
- DB3.1, J23101 and LigA were ented in liquid culture and grown overnight to make glycerol stocks and competent cells
