Template:Team:KULeuven/3 August 2009/VanillinProduction

From 2009.igem.org

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We received the ComT, Sam5 and Sam8 from Edinburgh today. They were put in competent cells by electroporation.
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We received the ''COMT, sam5'' and ''sam8'' from Edinburgh today. Unfortunately, the tube containing the ''sam5'' + RBS (with the PstI site) was opened during transport and all DNA in it was lost. The others were transferred into competent cells by electroporation.
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* Sam5 + RBS in pSB1A2 (with PstI site removed)
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* ''sam5'' + RBS in {{kulpart|pSB1A2}} (with PstI site removed), {{kulpart|BBa_K238007}}
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* Sam8 + RBS in pSB1A2
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* ''sam8'' + RBS in {{kulpart|pSB1A2}}, {{kulpart|BBa_I742106}}
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* Sam8 in pSB1A2 coding sequence, {{kulpart|BBa_I742142}}
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* ''sam8'' in {{kulpart|pSB1A2}} coding sequence, {{kulpart|BBa_I742142}}
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* ComT in pSB1A2 coding sequence, {{kulpart|BBa_I742107}}
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* ''COMT'' in {{kulpart|pSB1A2}} coding sequence, {{kulpart|BBa_I742107}}
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The cells were put in liquid LB to recuperate from the procedure and transfered onto LB/amp plates and grown overnight at 37°C.
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The cells were put into liquid LB to recuperate from the procedure, transferred onto LB/amp plates and grown overnight at 37°C.
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We also made liquid cultures of the RBS + ''fcs'' and RBS + ''ech'' cultures so we can miniprep them tomorrow.

Latest revision as of 09:46, 14 September 2009

We received the COMT, sam5 and sam8 from Edinburgh today. Unfortunately, the tube containing the sam5 + RBS (with the PstI site) was opened during transport and all DNA in it was lost. The others were transferred into competent cells by electroporation.

  • sam5 + RBS in (with PstI site removed),
  • sam8 + RBS in ,
  • sam8 in coding sequence,
  • COMT in coding sequence,

The cells were put into liquid LB to recuperate from the procedure, transferred onto LB/amp plates and grown overnight at 37°C.

We also made liquid cultures of the RBS + fcs and RBS + ech cultures so we can miniprep them tomorrow.