Template:Team:KULeuven/3 August 2009/VanillinProduction

From 2009.igem.org

(Difference between revisions)
 
(5 intermediate revisions not shown)
Line 1: Line 1:
-
We received the ComT, Sam5 and Sam8 from Edinburgh today. Unfortunately the tube containing the Sam5 + RBS (with the PstI site) had opened during transport and all DNA in it was lost. The others were put in competent cells by electroporation.
+
We received the ''COMT, sam5'' and ''sam8'' from Edinburgh today. Unfortunately, the tube containing the ''sam5'' + RBS (with the PstI site) was opened during transport and all DNA in it was lost. The others were transferred into competent cells by electroporation.
-
* Sam5 + RBS in pSB1A2 (with PstI site removed), {{kulpart|BBa_K238007}}
+
* ''sam5'' + RBS in {{kulpart|pSB1A2}} (with PstI site removed), {{kulpart|BBa_K238007}}
-
* Sam8 + RBS in pSB1A2
+
* ''sam8'' + RBS in {{kulpart|pSB1A2}}, {{kulpart|BBa_I742106}}
-
* Sam8 in pSB1A2 coding sequence, {{kulpart|BBa_I742142}}
+
* ''sam8'' in {{kulpart|pSB1A2}} coding sequence, {{kulpart|BBa_I742142}}
-
* ComT in pSB1A2 coding sequence, {{kulpart|BBa_I742107}}
+
* ''COMT'' in {{kulpart|pSB1A2}} coding sequence, {{kulpart|BBa_I742107}}
-
The cells were put in liquid LB to recuperate from the procedure and transfered onto LB/amp plates and grown overnight at 37°C.
+
The cells were put into liquid LB to recuperate from the procedure, transferred onto LB/amp plates and grown overnight at 37°C.
 +
 
 +
We also made liquid cultures of the RBS + ''fcs'' and RBS + ''ech'' cultures so we can miniprep them tomorrow.

Latest revision as of 09:46, 14 September 2009

We received the COMT, sam5 and sam8 from Edinburgh today. Unfortunately, the tube containing the sam5 + RBS (with the PstI site) was opened during transport and all DNA in it was lost. The others were transferred into competent cells by electroporation.

  • sam5 + RBS in (with PstI site removed),
  • sam8 + RBS in ,
  • sam8 in coding sequence,
  • COMT in coding sequence,

The cells were put into liquid LB to recuperate from the procedure, transferred onto LB/amp plates and grown overnight at 37°C.

We also made liquid cultures of the RBS + fcs and RBS + ech cultures so we can miniprep them tomorrow.