Template:Team:KULeuven/3 August 2009/VanillinProduction

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(New page: We received the ComT, Sam5 and Sam8 from Edinburgh today. They were put in competent cells by electroporation. * Sam5 + RBS in pSB1A2 (with PstI site removed) * Sam8 + RBS in pSB1A2 * Sam...)
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Revision as of 08:24, 4 August 2009

We received the ComT, Sam5 and Sam8 from Edinburgh today. They were put in competent cells by electroporation.

  • Sam5 + RBS in pSB1A2 (with PstI site removed)
  • Sam8 + RBS in pSB1A2
  • Sam8 in pSB1A2 coding sequence
  • ComT in pSB1A2 coding sequence

The cells were put in liquid LB to recuperate from the procedure and transfered onto LB/amp plates and grown overnight at 37°C.

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