Template:Team:KULeuven/3 September 2009/BlueLightReceptor

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  1. a restriction digest on with EcoRI and SpeI was performed. this will cut the promotor out of its vector. this vector () has a RFP reporter gene. we will put (cut with EcoRI and SpeI) in this vector. this way we will get a construct where RFP will be produced dependend on our blue light promotor. we can then compare this with the intensity of RFP produced by .
  2. glycerol stock was made from LigA and . they are stored in the -80°C in S&P F8 67-68 and 69-70.