Template:Team:KULeuven/6 September 2009/VanillinProduction

From 2009.igem.org

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** Transfered 4 colonies from EF ligation to a new plate
** Transfered 4 colonies from EF ligation to a new plate
** Put the 4 transfered colonies on liquid medium
** Put the 4 transfered colonies on liquid medium
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a miniprep and restrictie digest (ON) on sam5, sam8, ech and fcs was performed on saterday. on sunday, a gel was run to check the inserts. These were good so a motherplate for all the genes could be made.

Revision as of 08:19, 11 September 2009

  • Restriction digest overnight of Sam5 (1,2) and Sam8 (1,2)
  • Growth of EF Ligation (woohoo!)
    • Transfered 4 colonies from EF ligation to a new plate
    • Put the 4 transfered colonies on liquid medium

a miniprep and restrictie digest (ON) on sam5, sam8, ech and fcs was performed on saterday. on sunday, a gel was run to check the inserts. These were good so a motherplate for all the genes could be made.