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Template:Team:KULeuven/7 October 2009/BlueLightReceptor
From 2009.igem.org
(New page: experiment of 24/09 was repeated but the cultures were only grown and lit at 25°C. only the logA were used (in pBR322 vector) because its signal was clearer.) |
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| - | experiment of 24/09 was repeated but the cultures were only grown and lit at 25°C. only the logA were used (in pBR322 vector) because its signal was clearer. | + | experiment of 24/09 was repeated but the cultures were only grown and lit at 25°C. only the logA were used (in pBR322 vector) because its signal was clearer. In the previous measurement, we noticed that both the cells at 16°C and 25°C showed a higher GFP expression when irradiated with blue light, but we also noticed that because of the cold, the cultures grown at 16°C gave a higher GFP-expression even when they weren't irradiated. |
| + | That's why now, we only worked at Room temperature. Both growing overnight in the dark, and during the experiment, the cells were kept at room temperature. | ||
| + | We also noticed that a little cold shock could help our promotor start the GFP-production. The new overented cultures were made using a LB-medium kept at 10°C. This way, we obtained the best results. | ||
Revision as of 13:22, 20 October 2009
experiment of 24/09 was repeated but the cultures were only grown and lit at 25°C. only the logA were used (in pBR322 vector) because its signal was clearer. In the previous measurement, we noticed that both the cells at 16°C and 25°C showed a higher GFP expression when irradiated with blue light, but we also noticed that because of the cold, the cultures grown at 16°C gave a higher GFP-expression even when they weren't irradiated. That's why now, we only worked at Room temperature. Both growing overnight in the dark, and during the experiment, the cells were kept at room temperature. We also noticed that a little cold shock could help our promotor start the GFP-production. The new overented cultures were made using a LB-medium kept at 10°C. This way, we obtained the best results.
