UCL London/Protocol/Gel Electrophoresis
From 2009.igem.org
(Difference between revisions)
Line 7: | Line 7: | ||
: '''''Materials:''''' | : '''''Materials:''''' | ||
+ | |||
+ | * Agarose | ||
+ | * TAE buffer | ||
+ | * TAE buffer | ||
: '''''Method:''''' | : '''''Method:''''' | ||
+ | |||
+ | # Weigh 1.50g agarose, and add 1×TAE to make up to 150mL. | ||
+ | # Cool down the agarose and add 15 µL ethidium bromide into the agrose. | ||
+ | # Pour the gel into the tray before it settles down. | ||
+ | # Spin the digested DNA for 5 sec. | ||
+ | # Add 5 µL orange loading buffer (glycerol & dye) to the lid; spin for few sec. | ||
+ | # Cover the gel by TAE. | ||
+ | # Load the gel and note down the sequence. | ||
+ | # At 110V, running for 30 min (or 1hr depends on the equipment). | ||
+ | # Check with UV light & take picture. | ||
+ | |||
+ | |||
</div> | </div> | ||
{{Template:UCL_London_footer}} | {{Template:UCL_London_footer}} |
Latest revision as of 10:34, 18 October 2009
Gel Electrophoresis
- Materials:
- Agarose
- TAE buffer
- TAE buffer
- Method:
- Weigh 1.50g agarose, and add 1×TAE to make up to 150mL.
- Cool down the agarose and add 15 µL ethidium bromide into the agrose.
- Pour the gel into the tray before it settles down.
- Spin the digested DNA for 5 sec.
- Add 5 µL orange loading buffer (glycerol & dye) to the lid; spin for few sec.
- Cover the gel by TAE.
- Load the gel and note down the sequence.
- At 110V, running for 30 min (or 1hr depends on the equipment).
- Check with UV light & take picture.