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Uppsala-Sweden/11 October 2009
From 2009.igem.org
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==Plasmid prep of PRPRAT and PRKRYT== | ==Plasmid prep of PRPRAT and PRKRYT== | ||
| - | data shown here:[[Media:091011_Plasmidprep.xls]] | + | |
| + | data shown here: [[Media:091011_Plasmidprep.xls]] | ||
==Triparental mating== | ==Triparental mating== | ||
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Today we put our antisense-RNA constructs in to Synecocystis via triparental mating. | Today we put our antisense-RNA constructs in to Synecocystis via triparental mating. | ||
| - | Protocol here:[[Media:Triparental+Mating.txt]] | + | Protocol here: [[Media:Triparental+Mating.txt]] |
Deviated in that we did not concentrate the Synecocystis culture and instead just it straight and at 10 times dilution, corresponding to 10 and 100 times dilution in the protocol. | Deviated in that we did not concentrate the Synecocystis culture and instead just it straight and at 10 times dilution, corresponding to 10 and 100 times dilution in the protocol. | ||
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{{Uppsala-Sweden_Footer}} | {{Uppsala-Sweden_Footer}} | ||
Revision as of 15:59, 21 October 2009
Plasmid prep of PRPRAT and PRKRYT
data shown here: Media:091011_Plasmidprep.xls
Triparental mating
Today we put our antisense-RNA constructs in to Synecocystis via triparental mating. Protocol here: Media:Triparental+Mating.txt
Deviated in that we did not concentrate the Synecocystis culture and instead just it straight and at 10 times dilution, corresponding to 10 and 100 times dilution in the protocol.
