Uppsala-Sweden/11 October 2009

From 2009.igem.org

Revision as of 15:59, 21 October 2009 by Flormane (Talk | contribs)




Plasmid prep of PRPRAT and PRKRYT

data shown here: Media:091011_Plasmidprep.xls

Triparental mating

Today we put our antisense-RNA constructs in to Synecocystis via triparental mating. Protocol here: Media:Triparental+Mating.txt

Deviated in that we did not concentrate the Synecocystis culture and instead just it straight and at 10 times dilution, corresponding to 10 and 100 times dilution in the protocol.





Bioneer Biolegio Clontech Uppsala Genome Center

Retrieved from "http://2009.igem.org/Uppsala-Sweden/11_October_2009"