Uppsala-Sweden/15 September 2009

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Revision as of 16:14, 16 September 2009 by Flormane (Talk | contribs)




Picking colonies and colony PCR

The colonies from the plated transformations sice yesterday was picked this morning, together with the antisense RNA's that didn't seemed to have worked according to the evaluation gel from yesterday.

pPetE-E1A1-T, pPetE-E1B1-T, pPetE-E1B3-T, pPetE-E21-T, kivd, RBS-pdc-RBS-adh2z, RBS-kivd, kivd-RBS-YADH2-T, pPetE-RBS and RBS-PirA-RBS-PirB-T.

These colonies were run through a Taq PCR. Colony pcr 090915 pPR RK aRNA.jpg RK = Rbs-Kivd, RPRA = Rbs-Pdc-Rbs-Adh2Z, RART = Rbs-PirA-Rbs-PirB, KRYT = Kivd-Rbs-YAdh2-Ter Colony pcr 090915 RPRA RARBT KRYT.jpg

Some strange extra bands, probably due to annealing of the verification primers to the RBS as they share a high sequnce simalarity.

--Flormane 12:54, 15 September 2009 (UTC)

Gel Colony PCR of GFP and Lux System Parts

Ai: A340620 + i13504 (2,1kb) AJ: A340620 + J37034 (2,8kb) ACO: A340620 + C0261 (1,9kb) AB: A340620 + B0034 (1,2kb) Pi: pPetE + i13504 (1,6kb)

20090915 GFP LUX ColonyPCR-labelled.jpg

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