Virginia Commonwealth/10 July 2009

From 2009.igem.org

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==Friday 10 July 2009==
==Friday 10 July 2009==
===Results===
===Results===
''Afton and Maria''
''Afton and Maria''
*Overnight culture was successful
*Overnight culture was successful
 +
* Miniprep/Spectrophotometry Results
 +
{| cellpadding="5" border="1" padding="5"
 +
|+
 +
|-
 +
! Part No.!! A260 (nm) !! A280 (nm) !! A260/A280 !!  Vol. (µL) !! Conc. (µg/mL) !! Amt (µg)
 +
|-
 +
| J23106 || 0.015 || 0.004 || 3.750 || 120 || 22.5 || 2.700
 +
|-
 +
| J06702 || 0.022 || 0.013 || 1.692 || 164 || 33.0 || 5.412
 +
|-
 +
| J23100 || 0.036 || 0.025 || 1.440 || 54 || 22.5 || 2.808
 +
|}
 +
[[User:Trentay|Trentay]] 13:13, 10 July 2009 (UTC)
[[User:Trentay|Trentay]] 13:13, 10 July 2009 (UTC)
 +
 +
''Craig and Clay''
 +
* The overnight culture of the three parts related to limonene synthesis were unsuccessful.  Possible reasons include bad parts, errors in electrotransformation procedure, and incorrect resistance. 
----
----
Line 10: Line 64:
* Make stocks of parts J23106 and J06702
* Make stocks of parts J23106 and J06702
* Miniprep of parts J23106 and J06702
* Miniprep of parts J23106 and J06702
-
* Digestion of parts J23106 and J06702
+
* Digestion of parts J23106 and J06702, (and J23100)
* Electrophoresis test of parts J23106 and J06702
* Electrophoresis test of parts J23106 and J06702
* May Ligate parts with backbones pSB1C3 and pSB4C5 and store DNA
* May Ligate parts with backbones pSB1C3 and pSB4C5 and store DNA
Line 17: Line 71:
** Write out a plan to organize ideas
** Write out a plan to organize ideas
[[User:Trentay|Trentay]] 13:13, 10 July 2009 (UTC)
[[User:Trentay|Trentay]] 13:13, 10 July 2009 (UTC)
 +
 +
[[User:Trentay|Trentay]] 17:05, 13 August 2009 (UTC)
 +
 +
''Craig and Clay''
 +
* BBa_I742111, BBa_K118024, and BBa_K118025 need to be plated again.
 +
* If the new plates also show no growth, electrotransformation of the parts will be performed again.
 +
* If this proves to be unsuccessful, the cells from this second electrotransformation will also be plated a second time.  The cells will also be plated on non-resistant media.
 +
* If growth is again unsuccessful, the cells grown on the non-resistant media will be streaked on kanamycin and chloramphenicol plates.
----
----
====Wetlab====
====Wetlab====
Line 31: Line 93:
**Amount of DNA was calculated with a Spectrophotometer
**Amount of DNA was calculated with a Spectrophotometer
* Digestion was performed on MiniPrepped parts
* Digestion was performed on MiniPrepped parts
-
[[User:Trentay|Trentay]] 18:56, 10 July 2009 (UTC)
+
**There are two digested J06702 PCR tubes
 +
**Digests will be frozen until Monday
 +
***Electrophoresis test will be held off until then
 +
***Ligation as well
 +
[[User:Trentay|Trentay]] 20:35, 10 July 2009 (UTC)
 +
 
 +
''Craig and Clay''
 +
* The electrotransformed parts were plated again (ampicillin plates) due to unsuccessful growth.  Fifty microliters was used instead of 35 microliters.

Latest revision as of 17:13, 13 August 2009

May
MTWTFSS
        [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/1_May_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/2_May_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/3_May_2009&action=edit 3]
[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_May_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_May_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/6_May_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/7_May_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/8_May_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/9_May_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/10_May_2009&action=edit 10]
[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_May_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/12_May_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/13_May_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/14_May_2009&action=edit 14] [http://2009.igem.org/Virginia_Commonwealth/15_May_2009 15] [http://2009.igem.org/Virginia_Commonwealth/16_May_2009 16] [http://2009.igem.org/Virginia_Commonwealth/17_May_2009 17]
[http://2009.igem.org/Virginia_Commonwealth/18_May_2009 18] [http://2009.igem.org/Virginia_Commonwealth/19_May_2009 19] [http://2009.igem.org/Virginia_Commonwealth/20_May_2009 20] [http://2009.igem.org/Virginia_Commonwealth/21_May_2009 21] [http://2009.igem.org/Virginia_Commonwealth/22_May_2009 22] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/23_May_2009&action=edit 23] [http://2009.igem.org/Virginia_Commonwealth/24_May_2009 24]
[http://2009.igem.org/Virginia_Commonwealth/25_May_2009 25] [http://2009.igem.org/Virginia_Commonwealth/26_May_2009 26] [http://2009.igem.org/Virginia_Commonwealth/27_May_2009 27] [http://2009.igem.org/Virginia_Commonwealth/28_May_2009 28] [http://2009.igem.org/Virginia_Commonwealth/29_May_2009 29] [http://2009.igem.org/Virginia_Commonwealth/30_May_2009 30] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/31_May_2009&action=edit 31]
June
MTWTFSS
[http://2009.igem.org/Virginia_Commonwealth/1_June_2009 1] [http://2009.igem.org/Virginia_Commonwealth/2_June_2009 2] [http://2009.igem.org/Virginia_Commonwealth/3_June_2009 3] [http://2009.igem.org/Virginia_Commonwealth/4_June_2009 4] [http://2009.igem.org/Virginia_Commonwealth/5_June_2009 5] [http://2009.igem.org/Virginia_Commonwealth/6_June_2009 6] [http://2009.igem.org/Virginia_Commonwealth/7_June_2009 7]
[http://2009.igem.org/Virginia_Commonwealth/8_June_2009 8] [http://2009.igem.org/Virginia_Commonwealth/9_June_2009 9] [http://2009.igem.org/Virginia_Commonwealth/10_June_2009 10] [http://2009.igem.org/Virginia_Commonwealth/11_June_2009 11] [http://2009.igem.org/Virginia_Commonwealth/12_June_2009 12] [http://2009.igem.org/Virginia_Commonwealth/13_June_2009 13] [http://2009.igem.org/Virginia_Commonwealth/14_June_2009 14]
[http://2009.igem.org/Virginia_Commonwealth/15_June_2009 15] [http://2009.igem.org/Virginia_Commonwealth/16_June_2009 16] [http://2009.igem.org/Virginia_Commonwealth/17_June_2009 17] [http://2009.igem.org/Virginia_Commonwealth/18_June_2009 18] [http://2009.igem.org/Virginia_Commonwealth/19_June_2009 19] [http://2009.igem.org/Virginia_Commonwealth/20_June_2009 20] [http://2009.igem.org/Virginia_Commonwealth/21_June_2009 21]
[http://2009.igem.org/Virginia_Commonwealth/22_June_2009 22] [http://2009.igem.org/Virginia_Commonwealth/23_June_2009 23] [http://2009.igem.org/Virginia_Commonwealth/24_June_2009 24] [http://2009.igem.org/Virginia_Commonwealth/25_June_2009 25] [http://2009.igem.org/Virginia_Commonwealth/26_June_2009 26] [http://2009.igem.org/Virginia_Commonwealth/27_June_2009 27] [http://2009.igem.org/Virginia_Commonwealth/28_June_2009 28]
[http://2009.igem.org/Virginia_Commonwealth/29_June_2009 29] [http://2009.igem.org/Virginia_Commonwealth/30_June_2009 30]
July
MTWTFSS
    [http://2009.igem.org/Virginia_Commonwealth/1_July_2009 1] [http://2009.igem.org/Virginia_Commonwealth/2_July_2009 2] [http://2009.igem.org/Virginia_Commonwealth/3_July_2009 3] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_July_2009&action=edit 5]
[http://2009.igem.org/Virginia_Commonwealth/6_July_2009 6] [http://2009.igem.org/Virginia_Commonwealth/7_July_2009 7] [http://2009.igem.org/Virginia_Commonwealth/8_July_2009 8] [http://2009.igem.org/Virginia_Commonwealth/9_July_2009 9] [http://2009.igem.org/Virginia_Commonwealth/10_July_2009 10] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_July_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/12_July_2009&action=edit 12]
[http://2009.igem.org/Virginia_Commonwealth/13_July_2009 13] [http://2009.igem.org/Virginia_Commonwealth/14_July_2009 14] [http://2009.igem.org/Virginia_Commonwealth/15_July_2009 15] [http://2009.igem.org/Virginia_Commonwealth/16_July_2009 16] [http://2009.igem.org/Virginia_Commonwealth/17_July_2009 17] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/18_July_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/19_July_2009&action=edit 19]
[http://2009.igem.org/Virginia_Commonwealth/20_July_2009 20] [http://2009.igem.org/Virginia_Commonwealth/21_July_2009 21] [http://2009.igem.org/Virginia_Commonwealth/22_July_2009 22] [http://2009.igem.org/Virginia_Commonwealth/23_July_2009 23] [http://2009.igem.org/Virginia_Commonwealth/24_July_2009 24] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/25_July_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/26_July_2009&action=edit 26]
[http://2009.igem.org/Virginia_Commonwealth/27_July_2009 27] [http://2009.igem.org/Virginia_Commonwealth/28_July_2009 28] [http://2009.igem.org/Virginia_Commonwealth/29_July_2009 29] [http://2009.igem.org/Virginia_Commonwealth/30_July_2009 30] [http://2009.igem.org/Virginia_Commonwealth/31_July_2009 31]
August
MTWTFSS
          [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/1_August_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/2_August_2009&action=edit 2]
[http://2009.igem.org/Virginia_Commonwealth/3_August_2009 3] [http://2009.igem.org/Virginia_Commonwealth/4_August_2009 4] [http://2009.igem.org/Virginia_Commonwealth/5_August_2009 5] [http://2009.igem.org/Virginia_Commonwealth/6_August_2009 6] [http://2009.igem.org/Virginia_Commonwealth/7_August_2009 7] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/8_August_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/9_August_2009&action=edit 9]
[http://2009.igem.org/Virginia_Commonwealth/10_August_2009 10] [http://2009.igem.org/Virginia_Commonwealth/11_August_2009 11] [http://2009.igem.org/Virginia_Commonwealth/12_August_2009 12] [http://2009.igem.org/Virginia_Commonwealth/13_August_2009 13] [http://2009.igem.org/Virginia_Commonwealth/14_August_2009 14] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/15_August_2009&action=edit 15] [http://2009.igem.org/Virginia_Commonwealth/16_August_2009 16]
[http://2009.igem.org/Virginia_Commonwealth/17_August_2009 17] [http://2009.igem.org/Virginia_Commonwealth/18_August_2009 18] [http://2009.igem.org/Virginia_Commonwealth/19_August_2009 19] [http://2009.igem.org/Virginia_Commonwealth/20_August_2009 20] [http://2009.igem.org/Virginia_Commonwealth/21_August_2009 21] [http://2009.igem.org/Virginia_Commonwealth/22_August_2009 22] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/23_August_2009&action=edit 23]
[http://2009.igem.org/Virginia_Commonwealth/24_August_2009 24] [http://2009.igem.org/Virginia_Commonwealth/25_August_2009 25] [http://2009.igem.org/Virginia_Commonwealth/26_August_2009 26] [http://2009.igem.org/Virginia_Commonwealth/27_August_2009 27] [http://2009.igem.org/Virginia_Commonwealth/28_August_2009 28] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/29_August_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/30_August_2009&action=edit 30]
[http://2009.igem.org/Virginia_Commonwealth/31_August_2009 31]
September
MTWTFSS
  [http://2009.igem.org/Virginia_Commonwealth/1_September_2009 1] [http://2009.igem.org/Virginia_Commonwealth/2_September_2009 2] [http://2009.igem.org/Virginia_Commonwealth/3_September_2009 3] [http://2009.igem.org/Virginia_Commonwealth/4_September_2009 4] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_September_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/6_September_2009&action=edit 6]
[http://2009.igem.org/Virginia_Commonwealth/7_September_2009 7] [http://2009.igem.org/Virginia_Commonwealth/8_September_2009 8] [http://2009.igem.org/Virginia_Commonwealth/9_September_2009 9] [http://2009.igem.org/Virginia_Commonwealth/10_September_2009 10] [http://2009.igem.org/Virginia_Commonwealth/11_September_2009 11] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/12_September_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/13_September_2009&action=edit 13]
[http://2009.igem.org/Virginia_Commonwealth/14_September_2009 14] [http://2009.igem.org/Virginia_Commonwealth/15_September_2009 15] [http://2009.igem.org/Virginia_Commonwealth/16_September_2009 16] [http://2009.igem.org/Virginia_Commonwealth/17_September_2009 17] [http://2009.igem.org/Virginia_Commonwealth/18_September_2009 18] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/19_September_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/20_September_2009&action=edit 20]
[http://2009.igem.org/Virginia_Commonwealth/21_September_2009 21] [http://2009.igem.org/Virginia_Commonwealth/22_September_2009 22] [http://2009.igem.org/Virginia_Commonwealth/23_September_2009 23] [http://2009.igem.org/Virginia_Commonwealth/24_September_2009 24] [http://2009.igem.org/Virginia_Commonwealth/25_September_2009 25] [http://2009.igem.org/Virginia_Commonwealth/26_September_2009 26] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/27_September_2009&action=edit 27]
[http://2009.igem.org/Virginia_Commonwealth/28_September_2009 28] [http://2009.igem.org/Virginia_Commonwealth/29_September_2009 29] [http://2009.igem.org/Virginia_Commonwealth/30_September_2009 30]
October
MTWTFSS
      [http://2009.igem.org/Virginia_Commonwealth/1_October_2009 1] [http://2009.igem.org/Virginia_Commonwealth/2_October_2009 2] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/3_October_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_October_2009&action=edit 4]
[http://2009.igem.org/Virginia_Commonwealth/5_October_2009 5] [http://2009.igem.org/Virginia_Commonwealth/6_October_2009 6] [http://2009.igem.org/Virginia_Commonwealth/7_October_2009 7] [http://2009.igem.org/Virginia_Commonwealth/8_October_2009 8] [http://2009.igem.org/Virginia_Commonwealth/9_October_2009 9] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/10_October_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_October_2009&action=edit 11]
[http://2009.igem.org/Virginia_Commonwealth/12_October_2009 12] [http://2009.igem.org/Virginia_Commonwealth/13_October_2009 13] [http://2009.igem.org/Virginia_Commonwealth/14_October_2009 14] [http://2009.igem.org/Virginia_Commonwealth/15_October_2009 15] [http://2009.igem.org/Virginia_Commonwealth/16_October_2009 16] [http://2009.igem.org/Virginia_Commonwealth/17_October_2009 17] [http://2009.igem.org/Virginia_Commonwealth/18_October_2009 18]
[http://2009.igem.org/Virginia_Commonwealth/19_October_2009 19] [http://2009.igem.org/Virginia_Commonwealth/20_October_2009 20] [http://2009.igem.org/Virginia_Commonwealth/21_October_2009 21] [http://2009.igem.org/Virginia_Commonwealth/22_October_2009 22] [http://2009.igem.org/Virginia_Commonwealth/23_October_2009 23] [http://2009.igem.org/Virginia_Commonwealth/24_October_2009 24] [http://2009.igem.org/Virginia_Commonwealth/25_October_2009 25]
[http://2009.igem.org/Virginia_Commonwealth/26_October_2009 26] [http://2009.igem.org/Virginia_Commonwealth/27_October_2009 27] [http://2009.igem.org/Virginia_Commonwealth/28_October_2009 28] [http://2009.igem.org/Virginia_Commonwealth/29_October_2009 29] [http://2009.igem.org/Virginia_Commonwealth/30_October_2009 30] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/31_October_2009&action=edit 31]

Contents

Friday 10 July 2009

Results

Afton and Maria

  • Overnight culture was successful
  • Miniprep/Spectrophotometry Results
Part No. A260 (nm) A280 (nm) A260/A280 Vol. (µL) Conc. (µg/mL) Amt (µg)
J23106 0.015 0.004 3.750 120 22.5 2.700
J06702 0.022 0.013 1.692 164 33.0 5.412
J23100 0.036 0.025 1.440 54 22.5 2.808

Trentay 13:13, 10 July 2009 (UTC)

Craig and Clay

  • The overnight culture of the three parts related to limonene synthesis were unsuccessful. Possible reasons include bad parts, errors in electrotransformation procedure, and incorrect resistance.

Tasks

Afton and Maria

  • Make stocks of parts J23106 and J06702
  • Miniprep of parts J23106 and J06702
  • Digestion of parts J23106 and J06702, (and J23100)
  • Electrophoresis test of parts J23106 and J06702
  • May Ligate parts with backbones pSB1C3 and pSB4C5 and store DNA
  • Write out complete plan for experimentation
    • Make plans to MaxiPrep
    • Write out a plan to organize ideas

Trentay 13:13, 10 July 2009 (UTC)

Trentay 17:05, 13 August 2009 (UTC)

Craig and Clay

  • BBa_I742111, BBa_K118024, and BBa_K118025 need to be plated again.
  • If the new plates also show no growth, electrotransformation of the parts will be performed again.
  • If this proves to be unsuccessful, the cells from this second electrotransformation will also be plated a second time. The cells will also be plated on non-resistant media.
  • If growth is again unsuccessful, the cells grown on the non-resistant media will be streaked on kanamycin and chloramphenicol plates.

Wetlab

Afton and Maria

  • Dilution of overnight culture
    • 2 mL of overnight culture was added to 4 mL of LB broth (6 mL)
      • 2 dilutions were done for each test tube
    • Cells not used in the MiniPrep will be stored in the -80 degree Celsius freezer
  • 500mL of 30 percent Glycerol stock was made
  • Cell stocks were made
    • 10 vials of J06702 were put in the -80 degree Celsius freezer
    • 5 vials of J23106 were put in the -80 degree Celsius freezer
  • MiniPrep was done on parts: J23106 and J6702
    • Amount of DNA was calculated with a Spectrophotometer
  • Digestion was performed on MiniPrepped parts
    • There are two digested J06702 PCR tubes
    • Digests will be frozen until Monday
      • Electrophoresis test will be held off until then
      • Ligation as well

Trentay 20:35, 10 July 2009 (UTC)

Craig and Clay

  • The electrotransformed parts were plated again (ampicillin plates) due to unsuccessful growth. Fifty microliters was used instead of 35 microliters.