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Contents 1 Tuesday 28 July 2009 1.1 Results 1.2 Tasks 1.2.1 Wetlab

Tuesday 28 July 2009

Results

Maria and Afton

• Transformation
  • bacteria are visible on plate (approximately 6 small colonies)
  • no florescence is visible to the naked eye
• Possible reasons for problems:
  • Promoter works fine: when grown up in overnight culture, RFP was expressed
  • Backbone is there b/c bacteria have Cm resistance and one colony glowed in the past
  • There is no way to see if the RET DNA is there besides running it on a gel
  • Possibly enzymes and buffers for digestion and ligation are not working properly because they have been thawed out and refrozen too many times

Trentay 15:58, 29 July 2009 (UTC)

Kevin & Adam

• Early in the morning there was only one small colony visible from yesterday's transformations. At noon there were several more on various plates. Plates will be left all day and possibly overnight before determining the success/failure of yesterday's transformation.

Bussingkm 15:53, 29 July 2009 (UTC)

---

Tasks

Maria and Afton

• Write out plan for Digestion/Ligation test to see if enzymes are working
• Grow up part (Promoter + RET) in overnight culture in LB+AMP selective broth

Trentay 20:47, 29 July 2009 (UTC)

---

Wetlab

Maria and Afton

• Overnight culture was performed

Trentay 20:47, 29 July 2009 (UTC)

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