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Contents 1 Tuesday 09 June 2009 1.1 Results 1.2 Tasks 1.2.1 Drylab

Tuesday 09 June 2009

Results

---

Tasks

---

• Discussion: gel electrophoresis, BioBrick assembly, and the engineering design process
  • Gel electrophoresis is a type of size variation chromatography. DNA is pulled through the gel plate via a voltage and current. Used to verify the length of BioBricks.
  • BioBrick assembly

• • Engineering design process

• Literature discussion (especially relating to systems biology)

Drylab

• Characterizing promoters: Characterize the same seven promoter from "Measuring the activity of BioBrick promoters using an in vivo reference standard" and characterizing seven more.
  • Process variables

E. coli strains	Reporters	Backbone	Carbon source	Growth time	Temp
stock strains and W3110	BBa_E0240	pSB3K3	LB and Glycerol	1-12 hours	30 C

• • Promoters to be tested

Characterized	Not characterized
BBa_J23113	BBa_J23100
BBa_J23116	BBa_J23103
BBa_J23150	BBa_J23104
BBa_J23151	BBa_J23105
BBa_J23102	BBa_J23106
BBa_R0040	BBa_J23107
BBa_R0011	BBa_J23108

--

Reference promoter

BBa_J23101

• The reporter sequence codes for an RBS, GFP, and a terminator.
• We need to somehow take into account the degradation rate of the GFP. I suspect that other reporters such as RFP or luciferases will have different degradation rates. I also suspect that these degradation rates are not constant and might possibly skew the results of the test. For example, as the concentration of the reporter protein increases in concentration the degradation rate of might increase. In order to characterize and standardize the promoters we must be sure that no other variable is affecting florescence of the reporter. If we can refine the method from the paper and improve upon or confirm the results might be able to publish the results. This would give a solid leg up at the iGEM competition and significantly contribute to the field.

-Bussingkm 23:56, 9 June 2009 (UTC)

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