Wisconsin-Madison/28 June 2009

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Calendar

April
M	T	W	T	F	S	S
		[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/1_April_2009&action=edit 1]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/2_April_2009&action=edit 2]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/3_April_2009&action=edit 3]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/4_April_2009&action=edit 4]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/5_April_2009&action=edit 5]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/6_April_2009&action=edit 6]	[http://2009.igem.org/Wisconsin-Madison/7_April_2009 7]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/8_April_2009&action=edit 8]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/9_April_2009&action=edit 9]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/10_April_2009&action=edit 10]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/11_April_2009&action=edit 11]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/12_April_2009&action=edit 12]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/13_April_2009&action=edit 13]	[http://2009.igem.org/Wisconsin-Madison/14_April_2009 14]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/15_April_2009&action=edit 15]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/16_April_2009&action=edit 16]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/17_April_2009&action=edit 17]	[http://2009.igem.org/Wisconsin-Madison/18_April_2009 18]	[http://2009.igem.org/Wisconsin-Madison/19_April_2009 19]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/20_April_2009&action=edit 20]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/21_April_2009&action=edit 21]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/22_April_2009&action=edit 22]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/23_April_2009&action=edit 23]	[http://2009.igem.org/Wisconsin-Madison/24_April_2009 24]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/25_April_2009&action=edit 25]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/26_April_2009&action=edit 26]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/27_April_2009&action=edit 27]	[http://2009.igem.org/Wisconsin-Madison/28_April_2009 28]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/29_April_2009&action=edit 29]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/30_April_2009&action=edit 30]

May
M	T	W	T	F	S	S
				[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/1_May_2009&action=edit 1]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/2_May_2009&action=edit 2]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/3_May_2009&action=edit 3]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/4_May_2009&action=edit 4]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/5_May_2009&action=edit 5]	[http://2009.igem.org/Wisconsin-Madison/6_May_2009 6]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/7_May_2009&action=edit 7]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/8_May_2009&action=edit 8]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/9_May_2009&action=edit 9]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/10_May_2009&action=edit 10]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/11_May_2009&action=edit 11]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/12_May_2009&action=edit 12]	[http://2009.igem.org/Wisconsin-Madison/13_May_2009 13]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/14_May_2009&action=edit 14]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/15_May_2009&action=edit 15]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/16_May_2009&action=edit 16]	[http://2009.igem.org/Wisconsin-Madison/17_May_2009 17]
[http://2009.igem.org/Wisconsin-Madison/18_May_2009 18]	[http://2009.igem.org/Wisconsin-Madison/19_May_2009 19]	[http://2009.igem.org/Wisconsin-Madison/20_May_2009 20]	[http://2009.igem.org/Wisconsin-Madison/21_May_2009 21]	[http://2009.igem.org/Wisconsin-Madison/22_May_2009 22]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/23_May_2009&action=edit 23]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/24_May_2009&action=edit 24]
[http://2009.igem.org/Wisconsin-Madison/25_May_2009 25]	[http://2009.igem.org/Wisconsin-Madison/26_May_2009 26]	[http://2009.igem.org/Wisconsin-Madison/27_May_2009 27]	[http://2009.igem.org/Wisconsin-Madison/28_May_2009 28]	[http://2009.igem.org/Wisconsin-Madison/29_May_2009 29]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/30_May_2009&action=edit 30]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/31_May_2009&action=edit 31]

June
M	T	W	T	F	S	S
[http://2009.igem.org/Wisconsin-Madison/1_June_2009 1]	[http://2009.igem.org/Wisconsin-Madison/2_June_2009 2]	[http://2009.igem.org/Wisconsin-Madison/3_June_2009 3]	[http://2009.igem.org/Wisconsin-Madison/4_June_2009 4]	[http://2009.igem.org/Wisconsin-Madison/5_June_2009 5]	[http://2009.igem.org/Wisconsin-Madison/6_June_2009 6]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/7_June_2009&action=edit 7]
[http://2009.igem.org/Wisconsin-Madison/8_June_2009 8]	[http://2009.igem.org/Wisconsin-Madison/9_June_2009 9]	[http://2009.igem.org/Wisconsin-Madison/10_June_2009 10]	[http://2009.igem.org/Wisconsin-Madison/11_June_2009 11]	[http://2009.igem.org/Wisconsin-Madison/12_June_2009 12]	[http://2009.igem.org/Wisconsin-Madison/13_June_2009 13]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/14_June_2009&action=edit 14]
[http://2009.igem.org/Wisconsin-Madison/15_June_2009 15]	[http://2009.igem.org/Wisconsin-Madison/16_June_2009 16]	[http://2009.igem.org/Wisconsin-Madison/17_June_2009 17]	[http://2009.igem.org/Wisconsin-Madison/18_June_2009 18]	[http://2009.igem.org/Wisconsin-Madison/19_June_2009 19]	[http://2009.igem.org/Wisconsin-Madison/20_June_2009 20]	[http://2009.igem.org/Wisconsin-Madison/21_June_2009 21]
[http://2009.igem.org/Wisconsin-Madison/22_June_2009 22]	[http://2009.igem.org/Wisconsin-Madison/23_June_2009 23]	[http://2009.igem.org/Wisconsin-Madison/24_June_2009 24]	[http://2009.igem.org/Wisconsin-Madison/25_June_2009 25]	[http://2009.igem.org/Wisconsin-Madison/26_June_2009 26]	[http://2009.igem.org/Wisconsin-Madison/27_June_2009 27]	[http://2009.igem.org/Wisconsin-Madison/28_June_2009 28]
[http://2009.igem.org/Wisconsin-Madison/29_June_2009 29]	[http://2009.igem.org/Wisconsin-Madison/30_June_2009 30]

July
M	T	W	T	F	S	S
		[http://2009.igem.org/Wisconsin-Madison/1_July_2009 1]	[http://2009.igem.org/Wisconsin-Madison/2_July_2009 2]	[http://2009.igem.org/Wisconsin-Madison/3_July_2009 3]	[http://2009.igem.org/Wisconsin-Madison/4_July_2009 4]	[http://2009.igem.org/Wisconsin-Madison/5_July_2009 5]
[http://2009.igem.org/Wisconsin-Madison/6_July_2009 6]	[http://2009.igem.org/Wisconsin-Madison/7_July_2009 7]	[http://2009.igem.org/Wisconsin-Madison/8_July_2009 8]	[http://2009.igem.org/Wisconsin-Madison/9_July_2009 9]	[http://2009.igem.org/Wisconsin-Madison/10_July_2009 10]	[http://2009.igem.org/Wisconsin-Madison/11_July_2009 11]	[http://2009.igem.org/Wisconsin-Madison/12_July_2009 12]
[http://2009.igem.org/Wisconsin-Madison/13_July_2009 13]	[http://2009.igem.org/Wisconsin-Madison/14_July_2009 14]	[http://2009.igem.org/Wisconsin-Madison/15_July_2009 15]	[http://2009.igem.org/Wisconsin-Madison/16_July_2009 16]	[http://2009.igem.org/Wisconsin-Madison/17_July_2009 17]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/18_July_2009&action=edit 18]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/19_July_2009&action=edit 19]
[http://2009.igem.org/Wisconsin-Madison/20_July_2009 20]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/21_July_2009&action=edit 21]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/22_July_2009&action=edit 22]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/23_July_2009&action=edit 23]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/24_July_2009&action=edit 24]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/25_July_2009&action=edit 25]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/26_July_2009&action=edit 26]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/27_July_2009&action=edit 27]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/28_July_2009&action=edit 28]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/29_July_2009&action=edit 29]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/30_July_2009&action=edit 30]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/31_July_2009&action=edit 31]

August
M	T	W	T	F	S	S
					[http://2009.igem.org/Wisconsin-Madison/1_August_2009 1]	[http://2009.igem.org/Wisconsin-Madison/2_August_2009 2]
[http://2009.igem.org/Wisconsin-Madison/3_August_2009 3]	[http://2009.igem.org/Wisconsin-Madison/4_August_2009 4]	[http://2009.igem.org/Wisconsin-Madison/5_August_2009 5]	[http://2009.igem.org/Wisconsin-Madison/6_August_2009 6]	[http://2009.igem.org/Wisconsin-Madison/7_August_2009 7]	[http://2009.igem.org/Wisconsin-Madison/8_August_2009 8]	[http://2009.igem.org/Wisconsin-Madison/9_August_2009 9]
[http://2009.igem.org/Wisconsin-Madison/10_August_2009 10]	[http://2009.igem.org/Wisconsin-Madison/11_August_2009 11]	[http://2009.igem.org/Wisconsin-Madison/12_August_2009 12]	[http://2009.igem.org/Wisconsin-Madison/13_August_2009 13]	[http://2009.igem.org/Wisconsin-Madison/14_August_2009 14]	[http://2009.igem.org/Wisconsin-Madison/15_August_2009 15]	[http://2009.igem.org/Wisconsin-Madison/16_August_2009 16]
[http://2009.igem.org/Wisconsin-Madison/17_August_2009 17]	[http://2009.igem.org/Wisconsin-Madison/18_August_2009 18]	[http://2009.igem.org/Wisconsin-Madison/19_August_2009 19]	[http://2009.igem.org/Wisconsin-Madison/20_August_2009 20]	[http://2009.igem.org/Wisconsin-Madison/21_August_2009 21]	[http://2009.igem.org/Wisconsin-Madison/22_August_2009 22]	[http://2009.igem.org/Wisconsin-Madison/23_August_2009 23]
[http://2009.igem.org/Wisconsin-Madison/24_August_2009 24]	[http://2009.igem.org/Wisconsin-Madison/25_August_2009 25]	[http://2009.igem.org/Wisconsin-Madison/26_August_2009 26]	[http://2009.igem.org/Wisconsin-Madison/27_August_2009 27]	[http://2009.igem.org/Wisconsin-Madison/28_August_2009 28]	[http://2009.igem.org/Wisconsin-Madison/29_August_2009 29]	[http://2009.igem.org/Wisconsin-Madison/30_August_2009 30]
[http://2009.igem.org/Wisconsin-Madison/31_August_2009 31]

September
M	T	W	T	F	S	S
	[http://2009.igem.org/Wisconsin-Madison/1_September_2009 1]	[http://2009.igem.org/Wisconsin-Madison/2_September_2009 2]	[http://2009.igem.org/Wisconsin-Madison/3_September_2009 3]	[http://2009.igem.org/Wisconsin-Madison/4_September_2009 4]	[http://2009.igem.org/Wisconsin-Madison/5_September_2009 5]	[http://2009.igem.org/Wisconsin-Madison/6_September_2009 6]
[http://2009.igem.org/Wisconsin-Madison/7_September_2009 7]	[http://2009.igem.org/Wisconsin-Madison/8_September_2009 8]	[http://2009.igem.org/Wisconsin-Madison/9_September_2009 9]	[http://2009.igem.org/Wisconsin-Madison/10_September_2009 10]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/11_September_2009&action=edit 11]	[http://2009.igem.org/Wisconsin-Madison/12_September_2009 12]	[http://2009.igem.org/Wisconsin-Madison/13_September_2009 13]
[http://2009.igem.org/Wisconsin-Madison/14_September_2009 14]	[http://2009.igem.org/Wisconsin-Madison/15_September_2009 15]	[http://2009.igem.org/Wisconsin-Madison/16_September_2009 16]	[http://2009.igem.org/Wisconsin-Madison/17_September_2009 17]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/18_September_2009&action=edit 18]	[http://2009.igem.org/Wisconsin-Madison/19_September_2009 19]	[http://2009.igem.org/Wisconsin-Madison/20_September_2009 20]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/21_September_2009&action=edit 21]	[http://2009.igem.org/Wisconsin-Madison/22_September_2009 22]	[http://2009.igem.org/Wisconsin-Madison/23_September_2009 23]	[http://2009.igem.org/Wisconsin-Madison/24_September_2009 24]	[http://2009.igem.org/Wisconsin-Madison/25_September_2009 25]	[http://2009.igem.org/Wisconsin-Madison/26_September_2009 26]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/27_September_2009&action=edit 27]
[http://2009.igem.org/Wisconsin-Madison/28_September_2009 28]	[http://2009.igem.org/Wisconsin-Madison/29_September_2009 29]	[http://2009.igem.org/Wisconsin-Madison/30_September_2009 30]

October
M	T	W	T	F	S	S
			[http://2009.igem.org/Wisconsin-Madison/1_October_2009 1]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/2_October_2009&action=edit 2]	[http://2009.igem.org/Wisconsin-Madison/3_October_2009 3]	[http://2009.igem.org/Wisconsin-Madison/4_October_2009 4]
[http://2009.igem.org/Wisconsin-Madison/5_October_2009 5]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/6_October_2009&action=edit 6]	[http://2009.igem.org/Wisconsin-Madison/7_October_2009 7]	[http://2009.igem.org/Wisconsin-Madison/8_October_2009 8]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/9_October_2009&action=edit 9]	[http://2009.igem.org/Wisconsin-Madison/10_October_2009 10]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/11_October_2009&action=edit 11]
[http://2009.igem.org/Wisconsin-Madison/12_October_2009 12]	[http://2009.igem.org/Wisconsin-Madison/13_October_2009 13]	[http://2009.igem.org/Wisconsin-Madison/14_October_2009 14]	[http://2009.igem.org/Wisconsin-Madison/15_October_2009 15]	[http://2009.igem.org/Wisconsin-Madison/16_October_2009 16]	[http://2009.igem.org/Wisconsin-Madison/17_October_2009 17]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/18_October_2009&action=edit 18]
[http://2009.igem.org/Wisconsin-Madison/19_October_2009 19]	[http://2009.igem.org/Wisconsin-Madison/20_October_2009 20]	[http://2009.igem.org/Wisconsin-Madison/21_October_2009 21]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/22_October_2009&action=edit 22]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/23_October_2009&action=edit 23]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/24_October_2009&action=edit 24]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/25_October_2009&action=edit 25]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/26_October_2009&action=edit 26]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/27_October_2009&action=edit 27]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/28_October_2009&action=edit 28]	[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/29_October_2009&action=edit 29]	[http://2009.igem.org/Wisconsin-Madison/30_October_2009 30]	[http://2009.igem.org/Wisconsin-Madison/31_October_2009 31]

June 28, 2009

Ex 17: GFP Regulation by ProU Promoter

Test 3: Results: Fail, problems with the Plate Reader (connected to simulator)

Ex 15: Inducing Various Modified (triple) E.Coli Strains

Induction: 1. Irrabinose - 0.8 – 1% g/100mL

2. Wait 30 minutes

3. 1mM conc. IPTG (100uL/100mL)

4. Layer of Dodecane to trap isopentenol

Placed 5 ml of sample to into fresh 100ml LB, 50uL Amp, 73.5uL Cm, 100uL Tet OD 600

Control / BL21 (DE3) / MG1655 (W) / MG1655 Delta Ara

.395 / .290 / .184 / .249

.364 / .246 / .174 / .211

.398 / .266 / .183 / .227

.425 (I) / .225 / .219 / .227

.564 / .240 / .214 / .229

.916 / .305 / .239 / .303

1.35 / .322 / .33 / .332

2.039 / .429 (I) / .427 (I) / .432 (I)

1.0087 / 1.048 / 1.086 / 1.0085

40uL IPTG solution? (0.002g/ culture)

Results: It took 2 hours to reach OD

Maybe IPTG and Arabinose kill cell

Maybe Arabinose slows maetablolis,

Note - Cultures grew extraordinarily fast – USE less see culture (2.5ml)

Ex 16: MiniPrep of Choline Colonies A,C

Ran on 1% Agarose Gel

Undigested (A, C), 1kb Ladder, Digested (A, C)

Pc: PEAMT cut

Pu: PEAMT uncut

Expected size: 4150bp

Cut with: Pst1

Ex 16:

Growth:

Streaked and Inoculated: multiple colonies from multiple plats for Miniprep tomorrow

Both iGEM Plasmid: pSB1A3 different digestions

P1 - cut Ecor1and Spe1

P2 - cut Xba1 and Pst1

Also transformed NudF an ProU from last ligation :

Ex; Absorbance and fluorescence taken, man

Autoclaving Medium:

- Standard is to fill bottle half way full

- It is OK to fill bottle full unless Agar

- Agar needs to be half, will bubble

- Better to make half bottle to avoid contamination of medium

Ex 10: Cyanobacteria

added 8mL to 2mL 6803 cultures to achieve 10mL cultures - NP