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Team:Imperial College London/Wetlab/Protocols/PromoterCharacterisation
From 2009.igem.org
Promoter Characterisation (PC)
Section
Assay
Overview and Aims
Promoter Characterisation
IPTG
Characterise Lac promoter by varying concentrations of IPTG
Determine the IPTG concentration that allows for maximal protein production while still being non-toxic to the cell