Team:TorontoMaRSDiscovery/3 September 2009

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September 3, 2009

  1. only 1+2 (1), (4) showed growth in C
  2. Miniprepped Enc+5, 1+2 (1), (4)
  3. Digested Enc+5 with E,P, 1+2 (1), (4) with E,S, 3+2a with X,P for 1.5h
  4. Ran digests and old Tet digest from Monday on a gel
    • Forgot to add plasmid to controls
    • Tet digest still good
    • Enc+5 digest doesn't have ~900bp band -> not confirmed
  5. Started overnight ligation of 1+2+3+2/Tet
  6. Started overnight cultures: Ampx1, Kx2 Enc+5 x3, Tet, BB7
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