Team:EPF-Lausanne/Results/Fusion

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Revision as of 13:04, 19 October 2009







Fusion of the LOV domain and the trpR

DNA-binding domain



Fusion

The first step in our computational study of the LOV domain was to fuse the 2 domains of interest in VMD. We were then able to visualize the different proteins tried by Sosnick. The working protein, that we call LovTAP is the result of the fusion at PHE22 of trpR and can be seen on the next video. The general LOV domain is in yellow. Please note the chromophore called Flavin (FMN) in red in the center of LOV2. The trpR dna binding domain is in orange and DNA in gray.

The fusion was made in VMD by aligning the alpha helix of both domains on the backbone of 3 residues. The secondary structure is quite strong and conserved, what makes this fusion realistic.

         Click here to see an example of code used in VMD for the fusion


We also modelized the other fusion tried by Sosnick.

  • @MET11
  • @ALA12
  • @GLU13
  • ...
  • @PHE22
  • ...
  • @LEU25

         Clear here to view the other fusions


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