Team:KU Seoul/Parts
From 2009.igem.org
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::'''Mercury sensor - Mercury inducing promoter''' Part:{{Part|BBa_I728456}} + amd gene({{Part|BBa_K271000}}) | ::'''Mercury sensor - Mercury inducing promoter''' Part:{{Part|BBa_I728456}} + amd gene({{Part|BBa_K271000}}) | ||
[[Image:amd_part.png]] | [[Image:amd_part.png]] | ||
- | :Cadmium is an | + | :Cadmium is an intense toxic heavy metal with no known biological function. At low cadmium concentrations, cells are able to adapt and resume growth after a period of stationary period. Promoter yodA is only transiently activated during cadmium-induced growth inhibition. |
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- | + | Aryl acylamidase (EC 3.5.1.13; AAA) is an enzyme that acts on the amide bond between aryl- and acyl groups. The most typical reaction is the hydrolysis of an anilide, producing a carboxylate and aniline, which is also reversible. Acetaminophen(Tyrenol) is an aryl acyl compound having amide bonds which is hydrolyzed by the enzyme, producing acetate and p-aminophenol. Because p-Aminophenol show brown color, aryl acylamidase gene can be considered as reporter gene. | |
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:'''Heavy-metal detector {{Part|BBa_K271001}}''' | :'''Heavy-metal detector {{Part|BBa_K271001}}''' | ||
- | :The promoter/operator region of the zntA gene of Escherichia coli | + | :The promoter/operator region of the zntA gene of Escherichia coli. It shows that Zn(II) is the primary inducer of expression of this Zn(II)/Cd(II) export gene. The promoter PzntA shows sequence similarities to the promoters of mercury resistance(mer) operons, including a long spacer region containing an inverted repeat sequence. |
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::[[Image:zinc_part.png]] | ::[[Image:zinc_part.png]] |
Revision as of 15:50, 21 October 2009
Part
Aryl acylamidase (EC 3.5.1.13; AAA) is an enzyme that acts on the amide bond between aryl- and acyl groups. The most typical reaction is the hydrolysis of an anilide, producing a carboxylate and aniline, which is also reversible. Acetaminophen(Tyrenol) is an aryl acyl compound having amide bonds which is hydrolyzed by the enzyme, producing acetate and p-aminophenol. Because p-Aminophenol show brown color, aryl acylamidase gene can be considered as reporter gene.
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