Team:Newcastle/Labwork/7 September 2009

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Revision as of 16:29, 8 September 2009

Lab Work - 07/09/09

Metal Sensing Team

Introduction

In last week's lab session, we had received some BBa_J33206 + pSB1A2 DNA in it's original form by Chris French (the person who submitted the BioBrick to the Parts Registry) as well as both PCR and Sequencing primers. Whilst we used the sequencing primers to determine the sequence of the midi-prepped BBa_J33206 DNA derived from the Parts Registry, we also attempted to tranform some DH5-alpha E. coli bacteria with the original BBa_J33206 BioBrick sent to us.

In today's lab session, the plates on which the transformed cells were placed will be examined for colonies; if colonies should be present then they will be used to inoculate LB media in preparation for mini-preps.





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