Team:Imperial College London/Wetlab/Protocols/Cellculture

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(Cell culture protocols)
(Cell culture protocols)
 
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| <b>CC1</b>: Miniprep
| <b>CC1</b>: Miniprep
| [[Team:Imperial_College_London/ |Link]]
| [[Team:Imperial_College_London/ |Link]]
-
|* Harvest modest amounts of genomic DNA<br>
+
| * Harvest smaller amounts of genomic DNA<br>
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
| <b>CC2</b>: Cellulase
| <b>CC2</b>: Cellulase
| [[Team:Imperial_College_London/ |Link]]
| [[Team:Imperial_College_London/ |Link]]
-
|*Harvest large amount of genomic DNA<br>
+
| * Harvest larger amount of genomic DNA<br>
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
| <b>CC3</b>: Cell competence
| <b>CC3</b>: Cell competence
| [[Team:Imperial_College_London/ |Link]]
| [[Team:Imperial_College_London/ |Link]]
-
| *Making cells more competent to taking up DNA <br>
+
| * Making cells more competent to taking up DNA <br>
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
| <b>CC4</b>: Ligation
| <b>CC4</b>: Ligation
| [[Team:Imperial_College_London/ |Link]]
| [[Team:Imperial_College_London/ |Link]]
-
| *Ligation of different DNA constructs together<<br>
+
| * Ligation of different DNA constructs together<<br>
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
| <b>CC5</b>: Transformation (BL21)
| <b>CC5</b>: Transformation (BL21)
| [[Team:Imperial_College_London/ |Link]]
| [[Team:Imperial_College_London/ |Link]]
-
| *Transformation into BL21 cells using electrical shock <<br>
+
| * Transformation into BL21 cells using electrical shock <<br>
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
| <b>CC6</b>: Transformation (Top10)
| <b>CC6</b>: Transformation (Top10)
| [[Team:Imperial_College_London/Wetlab/Protocols/Cellculture/TransTop10 |Transformation into Top10]]
| [[Team:Imperial_College_London/Wetlab/Protocols/Cellculture/TransTop10 |Transformation into Top10]]
-
| *Transformation into chemically competent Top10 cells <<br>
+
| * Transformation into chemically competent Top10 cells <<br>
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
| <b>CC7</b>: Make LB plates
| <b>CC7</b>: Make LB plates
| [[Team:Imperial_College_London/Wetlab/Protocols/Cellculture/LBPlates |Make LB plates]]
| [[Team:Imperial_College_London/Wetlab/Protocols/Cellculture/LBPlates |Make LB plates]]
-
| *Making plates of LB agar for cell culturing<br>
+
| * Making plates of LB agar for cell culturing<br>
-
 
+
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
| <b>CC7</b>: Extract DNA from registry
| <b>CC7</b>: Extract DNA from registry
| [[Team:Imperial_College_London/Wetlab/Protocols/ExtractingDNA |Extracting DNA]]
| [[Team:Imperial_College_London/Wetlab/Protocols/ExtractingDNA |Extracting DNA]]
-
| *Extracting the required DNA from the registry<br>
+
| * Extracting the required DNA from the registry<br>
 +
 
 +
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
 +
| <b>CC13</b>: Genome Prep for Restriction asay
 +
| [[Team:Imperial_College_London/Wetlab/Protocols/GenomePrep |Genome Prep]]
 +
| * Preperation of the genomic DNA for restriction digest assay.
 +
 
 +
 
|}
|}
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Latest revision as of 11:05, 30 September 2009

Cell culture protocols

Section Assay Overview and Aims
CC1: Miniprep Link * Harvest smaller amounts of genomic DNA
CC2: Cellulase Link * Harvest larger amount of genomic DNA
CC3: Cell competence Link * Making cells more competent to taking up DNA
CC4: Ligation Link * Ligation of different DNA constructs together<
CC5: Transformation (BL21) Link * Transformation into BL21 cells using electrical shock <
CC6: Transformation (Top10) Transformation into Top10 * Transformation into chemically competent Top10 cells <
CC7: Make LB plates Make LB plates * Making plates of LB agar for cell culturing
CC7: Extract DNA from registry Extracting DNA * Extracting the required DNA from the registry
CC13: Genome Prep for Restriction asay Genome Prep * Preperation of the genomic DNA for restriction digest assay.


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