Team:UNIPV-Pavia/Notebook/Week5May
From 2009.igem.org
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*All the 15 incubated plates showed colonies! | *All the 15 incubated plates showed colonies! | ||
Moreover, many UCBerkeley promoter family members showed red fluorescent colonies, according to the ranking of promoters' estimated strength (see http://partsregistry.org/Promoters/Catalog/Anderson). | Moreover, many UCBerkeley promoter family members showed red fluorescent colonies, according to the ranking of promoters' estimated strength (see http://partsregistry.org/Promoters/Catalog/Anderson). | ||
+ | |||
+ | <table width="100%"> | ||
+ | <tr valign="up"> | ||
+ | <td> | ||
+ | {| | ||
+ | |[[Image:pv_firstday_plates.jpg|thumb|300px|left|High quality Berkeley promoters and E0240 plates: high yield for all of them!]] | ||
+ | |} | ||
+ | </td> | ||
+ | <td> | ||
+ | {| | ||
+ | |[[Image:pv_j102_plate.jpg|thumb|300px|left|J23102 plate: the promoter is very strong and RFP expression could be seen in the red-coloured colonies. The same result was seen in the other strong Berkeley promoters]] | ||
+ | |} | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
*We picked one colony from each plate and infected 1 ml of LB + Amp. We incubated the inocula at 37°C, 220 rpm for 5 and 1/2 hours. | *We picked one colony from each plate and infected 1 ml of LB + Amp. We incubated the inocula at 37°C, 220 rpm for 5 and 1/2 hours. |
Revision as of 09:47, 20 June 2009
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Week from May 25th, to May 31st, 2009
May, 25th
- iGEM starts now!!!
- We planned to dedicate the first two weeks to prepare a glycerol stock for every BioBrick of interest for our project. The following weeks will be dedicated to the BioBrick assembly.
- DNA resuspension from iGEM 2009 plates. We resuspended all the high quality constitutive promoter family members from UCBerkeley collection and GFP protein generator:
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- We picked one colony from each plate and infected 1 ml of LB + Amp. We incubated the inocula at 37°C, 220 rpm for 5 and 1/2 hours.
- After 5 hours and 1/2 all the cultures were turbid and bacteria were in their exponential growth phase.
- Glycerol stocks for the grown cultures.
May, 27th
Experiment with Tecan F200
- Description
- Purpose:
- Materials & Methods
- Protocol
- Results