Team:PKU Beijing/Notebook/Assembly/Rencheng Gao

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Revision as of 21:03, 21 October 2009
 
Notebook > Assembly > Assembling : bistable-> pSB1A2 & pSB6A1

2009.10.1

10:00
Double-enzyme digestion:

Plasmid(bistable on pSB4K3)5μl
SpeI1μl
PstI1μl
Buffer H2μl
ddH2O11μl

14:00
Gel purification

16:00
Ligation:
Vector: Bistable
Insert: T7-CI(1H, 2M, 2I, 2G, 2K, 1J)
Ligase: 1μl
Buffer: 1μl

18:00
Transformation

2009.10.2

10:00
Bacteria culture:
Bistable-T7p-CI(1H, 2M, 2I, 2G, 5N, 1J)

24:00
Mini-prep:
Bistable-T7p-CI(1H, 2M, 2I, 2G, 2K, 1J)

2009.10.3

09:00
Double-enzyme digestion: Bistable-T7p-CI(1H, 2M, 2I, 2G, 2K, 1J)

16:00
Ligation:
Vector: Bistable on 1O and on 2K
Insert: T7p-CI(1H, 2M, 2I, 2G, 2K, 1J)

20:00
Transformation

2009.10.4

09:00
Bacteria culture:

1O-Bistable-T7p-CI-2K, 1O-Bistable-T7p-CI-2G, 1O-Bistable-T7p-CI-2M, 12K-Bistable-T7p-CI-2I, 12K-Bistable-T7p-CI-1H, 12K-Bistable-T7p-CI-1J



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