Team:PKU Beijing/Parts

From 2009.igem.org

 

Parts We Contributed This Year

Our team has submitted 7 libraries of well characterized and standardized AND GATE devices. We contributed several sensor devices and tens of generators or transcriptional units which function well. More statistical data about the parts we contributed this year are shown in Table 1 below.

Table 1. Statistical data about our standardlized parts
Part TypesCodingRNADeviceRegulatoryCompositeGeneratorReporterTranscriptional UnitPlasmid BackboneTotal
Number2165210748191182

Favourite Parts

We've well characterized and already submitted 4 favourite parts. These parts have acted as critical componets in our system modules.


-?-NameTypeDescriptionDesignerLength
 WBBa_K228000CodingT7ptag(T7polymerase with amber mutation)Lin Min2673
 WBBa_K228001RNASupD-tRNALin Min136
 WBBa_K228003DeviceBistable partShan Shen2938
 WBBa_K228004RegulatoryNahR( reverse) - salicylate promoterLin Min1052
 WBBa_K228009RegulatoryAraC protein(reversed sequence) and Pbad promoterGao Rencheng1279
 WBBa_K228258DeviceAND GATE AraC+SupD+Sal+RBS(B0033)+T7ptag Guosheng Zhang5455
 WBBa_K228260DeviceAND GATE AraC+SupD+Sal+RBS(J44001)+T7ptag Guosheng Zhang5459


All Parts

All of our 182 parts are shown in the table below. Most of them have been proved to function well in our project.


-?-NameTypeDescriptionDesignerLength
 WBBa_K228002CompositeK228000-B0015Lin Min2810
 WBBa_K228005CompositeAraC protein and Pbad promoterGao Rencheng1279
 WBBa_K228006GeneratorAraC regulatory protein generatorGao Rencheng1095
 WBBa_K228007GeneratorConstitutive AraC regulatory protein generatorGao Rencheng1141
 WBBa_K228008GeneratorAraC regulatory proteinGao Rencheng1141
 WBBa_K228010ReporterLuxR-LuxP-GFPLin Min1930
 WBBa_K228011GeneratorLuxR-LuxP-SupDLin Min1325
 WBBa_K228012GeneratorAraC-Pbad-SupDGao Rencheng1423
 WBBa_K228013Reporter(pSal PO) OR Gate - GFPLin Min2038
 WBBa_K228031GeneratorAraC regulatory protein generator Gao Rencheng1098
 WBBa_K228032Translational_UnitRBS and AraC coding sequenceGao Rencheng958
 WBBa_K228033Translational_UnitRBS and AraC coding sequenceGao Rencheng958
 WBBa_K228034Translational_UnitRBS and AraC coding sequenceGao Rencheng961
 WBBa_K228035Translational_UnitRBS and AraC coding sequenceGao Rencheng958
 WBBa_K228036Translational_UnitRBS and AraC coding sequenceGao Rencheng958
 WBBa_K228037Translational_UnitRBS and AraC coding sequenceGao Rencheng959
 ?BBa_K228038DeviceAND Gate LuxR-rbs(B0030)-T7ptag-AraC-SupDGao Rencheng5314
 ?BBa_K228039DeviceAND Gate LuxR-rbs(B0031)-T7ptag-AraC-SupDGao Rencheng5313
 ?BBa_K228040DeviceAND Gate LuxR-rbs(B0032)-T7ptag-AraC-SupDGao Rencheng5312
 ?BBa_K228041DeviceAND Gate LuxR-rbs(B0033)-T7ptag-AraC-SupDGao Rencheng5310
 ?BBa_K228042DeviceAND Gate LuxR-rbs(B0034)-T7ptag-AraC-SupDGao Rencheng5311
 ?BBa_K228043DeviceAND Gate LuxR-rbs(J44001)-T7ptag-AraC-SupDGao Rencheng5314
 ?BBa_K228044DeviceAND Gate LuxR-rbs(J61100)-T7ptag-AraC-SupDGao Rencheng5311
 ?BBa_K228045DeviceAND Gate LuxR-rbs(J61107)-T7ptag-AraC-SupDGao Rencheng5311
 ?BBa_K228046DeviceAND Gate LuxR-rbs(J61127)-T7ptag-AraC-SupDGao Rencheng5311
 WBBa_K228098ReporterT7 promoter+medium RBS+GFPShan Shen930
 WBBa_K228099ReporterT7 promoter+strong RBS+GFPShan Shen932
 WBBa_K228100CompositeSupD + terminatorShan Shen273
 WBBa_K228101GeneratorLacP(R0010) + RBS(B0030) + T7ptag(K228000) + terminator(B0015)Shan Shen3039
 WBBa_K228102GeneratorLacP(R0010) + RBS(B0032) + T7ptag(K228000) + terminator(B0015)Shan Shen3037
 WBBa_K228103GeneratorLacP(R0010) + RBS(B0034) + T7ptag(K228000) + terminator(B0015)Shan Shen3036
 WBBa_K228104GeneratorLacP(R0010) + RBS(J61100) + T7ptag(K228000) + terminator(B0015)Shan Shen3036
 WBBa_K228105GeneratorLacP(R0010) + RBS(J61107) + T7ptag(K228000) + terminator(B0015)Shan Shen3036
 WBBa_K228106GeneratorLacP(R0010) + RBS(J61127) + T7ptag(K228000) + terminator(B0015)Shan Shen3036
 WBBa_K228107GeneratorLacP(R0010) + RBS(J44001) + T7ptag(K228000) + terminator(B0015)Shan Shen3039
 WBBa_K228108GeneratorLacP(R0010) + RBS(B0033) + T7ptag(K228000) + terminator(B0015)Shan Shen3035
 WBBa_K228109GeneratorLacP(R0010) + RBS(B0031) + T7ptag(K228000) + terminator(B0015)Shan Shen3038
 ?BBa_K228110DeviceAND GATE AraC + SupD + LacP +RBS(J61100)+T7ptagShan Shen4604
 ?BBa_K228111DeviceAND GATE AraC + SupD + LacP +RBS(J61107)+T7ptagShan Shen4604
 ?BBa_K228112DeviceAND GATE AraC + SupD + LacP +RBS(J61127)+T7ptagShan Shen4604
 ?BBa_K228113DeviceAND GATE AraC + SupD + LacP +RBS(B0033)+T7ptagShan Shen4603
 ?BBa_K228114DeviceAND GATE AraC + SupD + LacP +RBS(JB0031)+T7ptagShan Shen4606
 ?BBa_K228115DeviceAND GATE AraC + SupD + LacP +RBS(B0034)+T7ptagShan Shen4604
 ?BBa_K228116DeviceAND GATE AraC + SupD + LacP +RBS(B0030)+T7ptagShan Shen4607
 ?BBa_K228117DeviceAND GATE AraC + SupD + LacP +RBS(B0032)+T7ptagShan Shen4605
 ?BBa_K228118DeviceAND GATE AraC + SupD + LacP +RBS(J44001)+T7ptagShan Shen4607
 ?BBa_K228119DeviceAND GATE LacP+RBS(B0030)+T7ptag+LuxP+SupDShan Shen4372
 ?BBa_K228120DeviceAND GATE LacP+RBS(B0032)+T7ptag+LuxP+SupDShan Shen4370
 ?BBa_K228121DeviceAND GATE LacP+RBS(B0034)+T7ptag+LuxP+SupDShan Shen4369
 ?BBa_K228122DeviceAND GATE LacP+RBS(J61100)+T7ptag+LuxP+SupDShan Shen4369
 ?BBa_K228123DeviceAND GATE LacP+RBS(J61107)+T7ptag+LuxP+SupDShan Shen4369
 ?BBa_K228124DeviceAND GATE LacP+RBS(J611127)+T7ptag+LuxP+SupDShan Shen4369
 ?BBa_K228125DeviceAND GATE LacP+RBS(J44001)+T7ptag+LuxP+SupDShan Shen4372
 ?BBa_K228126DeviceAND GATE LacP+RBS(B0033)+T7ptag+LuxP+SupDShan Shen4368
 ?BBa_K228127DeviceAND GATE LacP+RBS(B0031)+T7ptag+LuxP+SupDShan Shen4371
 WBBa_K228200GeneratorRBS->T7ptag->TerminatorGuosheng Zhang2831
 WBBa_K228201GeneratorRBS->T7ptag->TerminatorGuosheng Zhang2830
 WBBa_K228202GeneratorRBS->T7ptag->TerminatorGuosheng Zhang2829
 WBBa_K228203GeneratorRBS->T7ptag->TerminatorGuosheng Zhang2827
 WBBa_K228204GeneratorRBS->T7ptag->TerminatorGuosheng Zhang2828
 WBBa_K228205GeneratorRBS->T7ptag->TerminatorGuosheng Zhang2831
 WBBa_K228206GeneratorRBS->T7ptag->TerminatorGuosheng Zhang2828
 WBBa_K228207GeneratorRBS->T7ptag->TerminatorGuosheng Zhang2828
 WBBa_K228208GeneratorRBS->T7ptag->TerminatorGuosheng Zhang2828
 WBBa_K228209GeneratorAraC->rbs->T7ptag->TerminatorGuosheng Zhang4118
 WBBa_K228210GeneratorAraC->rbs->T7ptag->TerminatorGuosheng Zhang4117
 WBBa_K228211GeneratorAraC->rbs->T7ptag->TerminatorGuosheng Zhang4116
 WBBa_K228212GeneratorAraC->rbs->T7ptag->TerminatorGuosheng Zhang4114
 WBBa_K228213GeneratorAraC->rbs->T7ptag->TerminatorGuosheng Zhang4115
 WBBa_K228214GeneratorAraC->rbs->T7ptag->TerminatorGuosheng Zhang4118
 WBBa_K228215GeneratorAraC->rbs->T7ptag->TerminatorGuosheng Zhang4115
 WBBa_K228216GeneratorAraC->rbs->T7ptag->TerminatorGuosheng Zhang4115
 WBBa_K228217GeneratorAraC->rbs->T7ptag->TerminatorGuosheng Zhang4115
 WBBa_K228218GeneratorLuxR->rbs->T7ptag->TerminatorGuosheng Zhang3883
 WBBa_K228219GeneratorLuxR->rbs->T7ptag->TerminatorGuosheng Zhang3882
 WBBa_K228220GeneratorLuxR->rbs->T7ptag->TerminatorGuosheng Zhang3881
 WBBa_K228221GeneratorLuxR->rbs->T7ptag->TerminatorGuosheng Zhang3879
 WBBa_K228222GeneratorLuxR->rbs->T7ptag->TerminatorGuosheng Zhang3880
 WBBa_K228223GeneratorLuxR->rbs->T7ptag->TerminatorGuosheng Zhang3883
 WBBa_K228224GeneratorLuxR->rbs->T7ptag->TerminatorGuosheng Zhang3880
 WBBa_K228225GeneratorLuxR->rbs->T7ptag->TerminatorGuosheng Zhang3880
 WBBa_K228226GeneratorLuxR->rbs->T7ptag->TerminatorGuosheng Zhang3880
 WBBa_K228227GeneratorSal->rbs->T7ptag->TerminatorGuosheng Zhang3891
 WBBa_K228228GeneratorSal->rbs->T7ptag->TerminatorGuosheng Zhang3890
 WBBa_K228229GeneratorSal->rbs->T7ptag->TerminatorGuosheng Zhang3889
 WBBa_K228230GeneratorSal->rbs->T7ptag->TerminatorGuosheng Zhang3887
 WBBa_K228231GeneratorSal->rbs->T7ptag->TerminatorGuosheng Zhang3888
 WBBa_K228232GeneratorSal->rbs->T7ptag->TerminatorGuosheng Zhang3891
 WBBa_K228233GeneratorSal->rbs->T7ptag->TerminatorGuosheng Zhang3888
 WBBa_K228234GeneratorSal->rbs->T7ptag->TerminatorGuosheng Zhang3888
 WBBa_K228235GeneratorSal->rbs->T7ptag->TerminatorGuosheng Zhang3888
 WBBa_K228236CompositecI->TerminatorGuosheng Zhang912
 WBBa_K228237GeneratorRBS->cI->TerminatorGuosheng Zhang933
 WBBa_K228238GeneratorRBS->cI->TerminatorGuosheng Zhang932
 WBBa_K228239GeneratorRBS->cI->TerminatorGuosheng Zhang931
 WBBa_K228240GeneratorRBS->cI->TerminatorGuosheng Zhang929
 WBBa_K228241GeneratorRBS->cI->TerminatorGuosheng Zhang930
 WBBa_K228242GeneratorRBS->cI->TerminatorGuosheng Zhang933
 WBBa_K228243GeneratorRBS->cI->TerminatorGuosheng Zhang930
 WBBa_K228244GeneratorRBS->cI->TerminatorGuosheng Zhang930
 WBBa_K228245GeneratorRBS->cI->TerminatorGuosheng Zhang930
 WBBa_K228246GeneratorT7 promoter->RBS->cI->TerminatorGuosheng Zhang987
 WBBa_K228247GeneratorT7 promoter->RBS->cI->TerminatorGuosheng Zhang986
 WBBa_K228248GeneratorT7 promoter->RBS->cI->TerminatorGuosheng Zhang985
 WBBa_K228249GeneratorT7 promoter->RBS->cI->TerminatorGuosheng Zhang983
 WBBa_K228250GeneratorT7 promoter->RBS->cI->TerminatorGuosheng Zhang984
 WBBa_K228251GeneratorT7 promoter->RBS->cI->TerminatorGuosheng Zhang987
 WBBa_K228252GeneratorT7 promoter->RBS->cI->TerminatorGuosheng Zhang984
 WBBa_K228253GeneratorT7 promoter->RBS->cI->TerminatorGuosheng Zhang984
 WBBa_K228254GeneratorT7 promoter->RBS->cI->TerminatorGuosheng Zhang984
 WBBa_K228255DeviceAND GATE AraC+SupD+Sal+RBS(B0030)+T7ptagGuosheng Zhang5459
 WBBa_K228256DeviceAND GATE AraC+SupD+Sal+RBS(B0031)+T7ptagGuosheng Zhang5458
 WBBa_K228257DeviceAND GATE AraC+SupD+Sal+RBS(B0032)+T7ptagGuosheng Zhang5457
 WBBa_K228259DeviceAND GATE AraC+SupD+Sal+RBS(B0034)+T7ptagGuosheng Zhang5456
 WBBa_K228261DeviceAND GATE AraC+SupD+Sal+RBS(J61100)+T7ptag Guosheng Zhang5456
 WBBa_K228262DeviceAND GATE AraC+SupD+Sal+RBS(J61107)+T7ptag Guosheng Zhang5456
 WBBa_K228263DeviceAND GATE AraC+SupD+Sal+RBS(J61127)+T7ptagGuosheng Zhang5456
 ?BBa_K228801Translational_UnitRBS(B0030)+lacI(C0012)Shuke Wu1174
 ?BBa_K228802Translational_UnitRBS(B0032)+lacI(C0012)Shuke Wu1172
 ?BBa_K228803Translational_UnitRBS(B0034)+lacI(C0012)Shuke Wu1171
 ?BBa_K228804Translational_UnitRBS(J61100)+lacI(C0012)Shuke Wu1171
 ?BBa_K228805Translational_UnitRBS(J61107)+lacI(C0012)Shuke Wu1171
 ?BBa_K228806Translational_UnitRBS(J61127)+lacI(C0012)Shuke Wu1171
 ?BBa_K228807Translational_UnitRBS(B0030)+tetR(C0040)Shuke Wu706
 ?BBa_K228808Translational_UnitRBS(B0032)+tetR(C0040)Shuke Wu704
 ?BBa_K228809Translational_UnitRBS(B0034)+tetR(C0040)Shuke Wu703
 ?BBa_K228810Translational_UnitRBS(J61100)+tetR(C0040)Shuke Wu703
 ?BBa_K228811Translational_UnitRBS(J61107)+tetR(C0040)Shuke Wu703
 ?BBa_K228812Translational_UnitRBS(J61127)+tetR(C0040)Shuke Wu703
 ?BBa_K228813GeneratorRBS(B0034)+lacI(C0012)+terminator(B0015)Shuke Wu1308
 ?BBa_K228814GeneratorRBS(B0034)+tetR(C0040)+terminator(B0015)Shuke Wu840
 ?BBa_K228815GeneratorPromoter(constitutive)+RBS(B0034)+lacI(C0012)+terminator(B0015)Shuke Wu1354
 ?BBa_K228816GeneratorPromoter(constitutive)+RBS(B0034)+tetR(C0040)+terminator(B0015)Shuke Wu886
 ?BBa_K228817CompositePcat+RBS(B0034)+lacI(C0012)+terminator(B0015)+lacP(R0010)Shuke Wu1562
 ?BBa_K228818CompositePcat+RBS(B0034)+tetR(C0040)+terminator(B0015)+tetP(R0040)Shuke Wu948
 ?BBa_K228819CompositePcat+RBS(B0034)+lacI(C0012)+term(B0015)+lacP(R0010)+GFPShuke Wu2448
 ?BBa_K228820CompositePcat+RBS(B0034)+tetR(C0040)+term(B0015)+tetP(R0040)+GFPShuke Wu1834
 WBBa_K228821ReporterlacP(R0010)+GFP(E0840)Shuke Wu1086
 WBBa_K228822CompositelacP(R0010)+SupD(K228001)+terminator(B0015) Shuke Wu481
 WBBa_K228823DeviceAND GATE lacP+SupD+AraC+RBS(J44001)+T7ptag Shuke Wu4607
 ?BBa_K228824DeviceAND GATE lacP+SupD+AraC+RBS(B0032)+T7ptag Shuke Wu4605
 ?BBa_K228825DeviceAND GATE lacP+SupD+AraC+RBS(J61127)+T7ptag Shuke Wu4604
 ?BBa_K228826DeviceAND GATE lacP+SupD+AraC+RBS(J61107)+T7ptag Shuke Wu4604
 ?BBa_K228827DeviceAND GATE lacP+SupD+AraC+RBS(B0030)+T7ptag Shuke Wu4607
 ?BBa_K228828DeviceAND GATE lacP+SupD+AraC+RBS(J61100)+T7ptag Shuke Wu4604
 WBBa_K228829DeviceAND GATE lacP+SupD+AraC+RBS(B0033)+T7ptag Shuke Wu4603
 ?BBa_K228830DeviceAND GATE lacP+SupD+AraC+RBS(B0031)+T7ptag Shuke Wu4606
 ?BBa_K228831DeviceAND GATE lacP+SupD+AraC+RBS(B0034)+T7ptag Shuke Wu4604
 WBBa_K228850ReporterPO-GFPLin Min978
 WBBa_K228851GeneratorSal+supD+terminatorHe Siheng1333
 ?BBa_K228852DeviceAND GATE Sal+SupD+LacP+RBS(J44001)+T7ptag He Siheng4380
 ?BBa_K228853DeviceAND GATE Sal+SupD+LacP+RBS(B0030)+T7ptag He Siheng4380
 ?BBa_K228854DeviceAND GATE Sal+SupD+LacP+RBS(B0032)+T7ptag He Siheng4378
 ?BBa_K228855DeviceAND GATE Sal+SupD+LacP+RBS(B0034)+T7ptag He Siheng4377
 ?BBa_K228856DeviceAND GATE Sal+SupD+LacP+RBS(J61100)+T7ptag He Siheng4377
 ?BBa_K228857DeviceAND GATE Sal+SupD+LacP+RBS(J61107)+T7ptag He Siheng4377
 ?BBa_K228858DeviceAND GATE Sal+SupD+LacP+RBS(J61127)+T7ptag He Siheng4377
 ?BBa_K228859DeviceAND GATE Sal+SupD+LacP+RBS(B0033)+T7ptag He Siheng4376
 ?BBa_K228860DeviceAND GATE Sal+SupD+LacP+RBS(B0031)+T7ptag He Siheng4379
 WBBa_K228861ReporterOR Gate-GFPLin Min2038
 WBBa_K228862ReportersalP-GFPHaoqian Zhang1938
 WBBa_K228863CodingPhiR73 delta with amber mutationLin Min243
 WBBa_K228864Translational_UnitRBS(B0034)-mutated PhiR73 delta(K228863)Lin Min261
 WBBa_K228865CompositepSal-rbs-PhiR73 ( amber mutation )Lin Min1321
 WBBa_K228866DeviceAND Gate pBad-SupD pSal-PhiR73 delta(amber mutation)Lin Min2752
 WBBa_K228867Plasmid_BackbonepSB1A2-lacZ screening plasmidLin Min2366
  BBa_K228868GeneratorAraC(K228009) + RBS(B0033) + T7ptag(K228000) + terminator(B0015)Haoqian Zhang4114
  BBa_K228869GeneratorAraC(K228009) + RBS(B0031) + T7ptag(K228000) + terminator(B0015)Haoqian Zhang4117
 ?BBa_K228870DeviceAND GATE AraC+RBS(B0030)+T7ptag+Sal+SupDHaoqian Zhang5459
 ?BBa_K228871DeviceAND GATE AraC+RBS(B0032)+T7ptag+Sal+SupDHaoqian Zhang5457
 ?BBa_K228872DeviceAND GATE AraC+RBS(B0034)+T7ptag+Sal+SupDHaoqian Zhang5456
 ?BBa_K228873DeviceAND GATE AraC+RBS(J61100)+T7ptag+Sal+SupDHaoqian Zhang5456
 ?BBa_K228874DeviceAND GATE AraC+RBS(J61107)+T7ptag+Sal+SupDHaoqian Zhang5456
 ?BBa_K228875DeviceAND GATE AraC+RBS(J61127)+T7ptag+Sal+SupDHaoqian Zhang5456
 ?BBa_K228876DeviceAND GATE AraC+RBS(J44001)+T7ptag+Sal+SupDHaoqian Zhang5459
 ?BBa_K228877DeviceAND GATE AraC+RBS(B0033)+T7ptag+Sal+SupDHaoqian Zhang5455
 ?BBa_K228878DeviceAND GATE AraC+RBS(B0031)+T7ptag+Sal+SupDHaoqian Zhang5458


Safety

Because this part is from commonly used elements in bacteria. No safety problem can be arised by this part.



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